Transient Cold Shock Enhances Zinc-finger Nuclease-mediated Gene Disruption

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 May 4

PMID 20436476

Citations 94

Authors

Yannick Doyon

Vivian M Choi

Danny F Xia

Thuy D Vo

Philip D Gregory

Michael C Holmes

Affiliations

Soon will be listed here.

Abstract

Zinc-finger nucleases (ZFNs) are powerful tools for editing the genomes of cell lines and model organisms. Given the breadth of their potential application, simple methods that increase ZFN activity, thus ensuring genome modification, are highly attractive. Here we show that transient hypothermia generally and robustly increased the level of stable, ZFN-induced gene disruption, thereby providing a simple technique to enhance the experimental efficacy of ZFNs.

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