HPLC-assisted Automated Oligosaccharide Synthesis

Overview

Journal Org Lett

Publisher American Chemical Society

Specialties Biochemistry
Chemistry

Date 2012 Jun 1

PMID 22646669

Citations 34

Authors

N Vijaya Ganesh

Kohki Fujikawa

Yih Horng Tan

Keith J Stine

Alexei V Demchenko

Affiliations

Soon will be listed here.

Abstract

A standard HPLC was adapted to polymer supported oligosaccharide synthesis. Solution-based reagents are delivered using a software-controlled solvent delivery system. The reaction progress and completion can be monitored in real time using a standard UV detector. All steps of oligosaccharide assembly including loading, glycosylation, deprotection, and cleavage can be performed using this setup.

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