An Automated Platform for the Enzyme-mediated Assembly of Complex Oligosaccharides

Overview

Journal Nat Chem

Specialty Chemistry

Date 2019 Feb 23

PMID 30792508

Citations 54

Authors

Tiehai Li

Lin Liu

Na Wei

Jeong-Yeh Yang

Digantkumar G Chapla

Kelley W Moremen

Geert-Jan Boons

Affiliations

Soon will be listed here.

Abstract

An automated platform that can synthesize a wide range of complex carbohydrates will greatly increase their accessibility and should facilitate progress in glycoscience. Here we report a fully automated process for enzyme-mediated oligosaccharide synthesis that can give easy access to different classes of complex glycans including poly-N-acetyllactosamine derivatives, human milk oligosaccharides, gangliosides and N-glycans. Our automated platform uses a catch and release approach in which glycosyltransferase-catalysed reactions are performed in solution and product purification is accomplished by solid phase extraction. We developed a sulfonate tag that can easily be installed and enables highly efficient solid phase extraction and product release using a single set of washing conditions, regardless of the complexity of the glycan. Using this custom-built synthesizer, as many as 15 reaction cycles can be performed in an automated fashion without a need for lyophilization or buffer exchange steps.

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