Photothermic Regulation of Gene Expression Triggered by Laser-induced Carbon Nanohorns

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2012 Apr 25

PMID 22529368

Citations 18

Authors

Eijiro Miyako

Tomonori Deguchi

Yoshihiro Nakajima

Masako Yudasaka

Yoshihisa Hagihara

Masanori Horie

Mototada Shichiri

Yuriko Higuchi

Fumiyoshi Yamashita

Mitsuru Hashida

Yasushi Shigeri

Yasukazu Yoshida

Sumio Iijima

Affiliations

Soon will be listed here.

Abstract

The development of optical methods to control cellular functions is important for various biological applications. In particular, heat shock promoter-mediated gene expression systems by laser light are attractive targets for controlling cellular functions. However, previous approaches have considerable technical limitations related to their use of UV, short-wavelength visible (vis), and infrared (IR) laser light, which have poor penetration into biological tissue. Biological tissue is relatively transparent to light inside the diagnostic window at wavelengths of 650-1,100 nm. Here we present a unique optical biotechnological method using carbon nanohorn (CNH) that transforms energy from diagnostic window laser light to heat to control the expression of various genes. We report that with this method, laser irradiation within the diagnostic window resulted in effective heat generation and thus caused heat shock promoter-mediated gene expression. This study provides an important step forward in the development of light-manipulated gene expression technologies.

Citing Articles

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