Optogenetic Control of Heart Muscle in Vitro and in Vivo

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2010 Oct 1

PMID 20881965

Citations 205

Authors

Tobias Bruegmann

Daniela Malan

Michael Hesse

Thomas Beiert

Christopher J Fuegemann

Bernd K Fleischmann

Philipp Sasse

Affiliations

Soon will be listed here.

Abstract

Electrical stimulation is the standard technique for exploring electrical behavior of heart muscle, but this approach has considerable technical limitations. Here we report expression of the light-activated cation channel channelrhodopsin-2 for light-induced stimulation of heart muscle in vitro and in mice. This method enabled precise localized stimulation and constant prolonged depolarization of cardiomyocytes and cardiac tissue resulting in alterations of pacemaking, Ca(2+) homeostasis, electrical coupling and arrhythmogenic spontaneous extrabeats.

Citing Articles

Optogenetic quantification of cardiac excitability and electrical coupling in intact hearts to explain cardiac arrhythmia initiation.

Langen J, Boyle P, Malan D, Sasse P Sci Adv. 2025; 11(9):eadt4103.

PMID: 40020054 PMC: 11870084. DOI: 10.1126/sciadv.adt4103.

Monitoring and modulating cardiac bioelectricity: from Einthoven to end-user.

De Coster T, Nobacht A, Oostendorp T, de Vries A, Coronel R, Pijnappels D Europace. 2024; 27(1).

PMID: 39716965 PMC: 11711590. DOI: 10.1093/europace/euae300.

Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids.

Guragain B, Zhang H, Wu Y, Wang Y, Wei Y, Wood G J Mol Cell Cardiol. 2024; 199:51-59.

PMID: 39674364 PMC: 11788028. DOI: 10.1016/j.yjmcc.2024.12.003.

Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review.

Baines O, Sha R, Kalla M, Holmes A, Efimov I, Pavlovic D Europace. 2024; 26(2).

PMID: 38227822 PMC: 10847904. DOI: 10.1093/europace/euae017.

Cardiac optogenetics: shining light on signaling pathways.

Leemann S, Schneider-Warme F, Kleinlogel S Pflugers Arch. 2023; 475(12):1421-1437.

PMID: 38097805 PMC: 10730638. DOI: 10.1007/s00424-023-02892-y.

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