Mucin Biopolymers As Broad-spectrum Antiviral Agents

Overview

Journal Biomacromolecules

Publisher American Chemical Society

Specialties Biochemistry
Biology
Molecular Biology

Date 2012 Apr 6

PMID 22475261

Citations 62

Authors

Oliver Lieleg

Corinna Lieleg

Jesse Bloom

Christopher B Buck

Katharina Ribbeck

Affiliations

Soon will be listed here.

Abstract

Mucus is a porous biopolymer matrix that coats all wet epithelia in the human body and serves as the first line of defense against many pathogenic bacteria and viruses. However, under certain conditions viruses are able to penetrate this infection barrier, which compromises the protective function of native mucus. Here, we find that isolated porcine gastric mucin polymers, key structural components of native mucus, can protect an underlying cell layer from infection by small viruses such as human papillomavirus (HPV), Merkel cell polyomavirus (MCV), or a strain of influenza A virus. Single particle analysis of virus mobility inside the mucin barrier reveals that this shielding effect is in part based on a retardation of virus diffusion inside the biopolymer matrix. Our findings suggest that purified mucins may be used as a broad-range antiviral supplement to personal hygiene products, baby formula or lubricants to support our immune system.

Citing Articles

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Cultivated meat microbiological safety considerations and practices.

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A mucus layer derived from porcine intestinal organoid air-liquid interface monolayer attenuates swine enteric coronavirus infection by antiviral activity of Muc2.

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Iron content of commercial mucin contributes to compositional stability of a cystic fibrosis airway synthetic microbiota community.

Giedraitis E, Neve R, Phelan V bioRxiv. 2024; .

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References

Vladescu I, Lieleg O, Jang S, Ribbeck K . An adsorption chromatography assay to probe bulk particle transport through hydrogels. J Pharm Sci. 2011; 101(1):436-42. PMC: 3650840. DOI: 10.1002/jps.22737. View

Loyo M, Guerrero-Preston R, Brait M, Hoque M, Chuang A, Kim M . Quantitative detection of Merkel cell virus in human tissues and possible mode of transmission. Int J Cancer. 2009; 126(12):2991-6. PMC: 2856748. DOI: 10.1002/ijc.24737. View

Lai S, Wang Y, Hida K, Cone R, Hanes J . Nanoparticles reveal that human cervicovaginal mucus is riddled with pores larger than viruses. Proc Natl Acad Sci U S A. 2009; 107(2):598-603. PMC: 2818964. DOI: 10.1073/pnas.0911748107. View

Schiller J, Day P, Kines R . Current understanding of the mechanism of HPV infection. Gynecol Oncol. 2010; 118(1 Suppl):S12-7. PMC: 3493113. DOI: 10.1016/j.ygyno.2010.04.004. View

Lai S, OHanlon D, Harrold S, Man S, Wang Y, Cone R . Rapid transport of large polymeric nanoparticles in fresh undiluted human mucus. Proc Natl Acad Sci U S A. 2007; 104(5):1482-7. PMC: 1785284. DOI: 10.1073/pnas.0608611104. View

Schiffman M, Castle P, Jeronimo J, Rodriguez A, Wacholder S . Human papillomavirus and cervical cancer. Lancet. 2007; 370(9590):890-907. DOI: 10.1016/S0140-6736(07)61416-0. View

Tian P, Brandl M, Mandrell R . Porcine gastric mucin binds to recombinant norovirus particles and competitively inhibits their binding to histo-blood group antigens and Caco-2 cells. Lett Appl Microbiol. 2005; 41(4):315-20. DOI: 10.1111/j.1472-765X.2005.01775.x. View

Varghese J, McKimm-Breschkin J, Caldwell J, Kortt A, Colman P . The structure of the complex between influenza virus neuraminidase and sialic acid, the viral receptor. Proteins. 1992; 14(3):327-32. DOI: 10.1002/prot.340140302. View

Bergey E, Gu M, Collins A, Bradway S, LeVine M . Modulation of herpes simplex virus type 1 replication by human salivary secretions. Oral Microbiol Immunol. 1993; 8(2):89-93. DOI: 10.1111/j.1399-302x.1993.tb00551.x. View

10.

Wieland U, Mauch C, Kreuter A, Krieg T, Pfister H . Merkel cell polyomavirus DNA in persons without merkel cell carcinoma. Emerg Infect Dis. 2009; 15(9):1496-8. PMC: 2819892. DOI: 10.3201/eid1509.081575. View

11.

Chittum H, Lane W, Carlson B, Roller P, Lung F, Lee B . Rabbit beta-globin is extended beyond its UGA stop codon by multiple suppressions and translational reading gaps. Biochemistry. 1998; 37(31):10866-70. DOI: 10.1021/bi981042r. View

12.

Mahalingam A, Jay J, Langheinrich K, Shukair S, McRaven M, Rohan L . Inhibition of the transport of HIV in vitro using a pH-responsive synthetic mucin-like polymer system. Biomaterials. 2011; 32(33):8343-55. PMC: 3183097. DOI: 10.1016/j.biomaterials.2011.05.001. View

13.

Roy A, Parker J, Parrish C, Whittaker G . Early stages of influenza virus entry into Mv-1 lung cells: involvement of dynamin. Virology. 2000; 267(1):17-28. DOI: 10.1006/viro.1999.0109. View

14.

Matrosovich M, Matrosovich T, Gray T, Roberts N, Klenk H . Neuraminidase is important for the initiation of influenza virus infection in human airway epithelium. J Virol. 2004; 78(22):12665-7. PMC: 525087. DOI: 10.1128/JVI.78.22.12665-12667.2004. View

15.

Crater J, Carrier R . Barrier properties of gastrointestinal mucus to nanoparticle transport. Macromol Biosci. 2010; 10(12):1473-83. DOI: 10.1002/mabi.201000137. View

16.

Buck C, Pastrana D, Lowy D, Schiller J . Efficient intracellular assembly of papillomaviral vectors. J Virol. 2003; 78(2):751-7. PMC: 368835. DOI: 10.1128/jvi.78.2.751-757.2004. View

17.

Kalluri R . Basement membranes: structure, assembly and role in tumour angiogenesis. Nat Rev Cancer. 2003; 3(6):422-33. DOI: 10.1038/nrc1094. View

18.

Neumann G, Noda T, Kawaoka Y . Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature. 2009; 459(7249):931-9. PMC: 2873852. DOI: 10.1038/nature08157. View

19.

Matsui H, Verghese M, Kesimer M, Schwab U, Randell S, Sheehan J . Reduced three-dimensional motility in dehydrated airway mucus prevents neutrophil capture and killing bacteria on airway epithelial surfaces. J Immunol. 2005; 175(2):1090-9. DOI: 10.4049/jimmunol.175.2.1090. View

20.

Yates 3rd J, Eng J, McCormack A, Schieltz D . Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database. Anal Chem. 1995; 67(8):1426-36. DOI: 10.1021/ac00104a020. View