An Adsorption Chromatography Assay to Probe Bulk Particle Transport Through Hydrogels

Overview

Journal J Pharm Sci

Publisher Elsevier

Specialties Pharmacology
Pharmacy

Date 2011 Sep 10

PMID 21905030

Citations 5

Authors

I Vladescu

O Lieleg

S Jang

Katharina Ribbeck

Affiliations

Soon will be listed here.

Abstract

Biopolymer-based hydrogels such as mucus and the basal lamina play a key role in biology, where they control the exchange of material between different compartments. They also pose a barrier that needs to be overcome for successful drug delivery. Characterizing the permeability properties of such hydrogels is mandatory for the development of suitable drug delivery vectors and pharmaceutics. Here, we present an experimental method to measure bulk particle transport through hydrogels. We validate our assay by applying it to mucin hydrogels and show that the permeability properties of these mucin hydrogels can be modulated by polymer density and pH, in agreement with previous results obtained from single particle tracking. The method we present here is easy to handle, inexpensive, and high-throughput compatible. It is also a suitable platform for the design and screening of drugs that aim at modifying the barrier properties of hydrogels. This system can also aid in the characterization and development of synthetic gels for a range of biomedical applications.

Citing Articles

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Replication of the Taste of Sugar by Formulation of Noncaloric Sweeteners with Mineral Salt Taste Modulator Compositions.

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Enzymatically active biomimetic micropropellers for the penetration of mucin gels.

Walker D, Kasdorf B, Jeong H, Lieleg O, Fischer P Sci Adv. 2016; 1(11):e1500501.

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Pediatric laryngotracheal reconstruction with tissue-engineered cartilage in a rabbit model.

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Mucin biopolymers as broad-spectrum antiviral agents.

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