Tracking the Progression of the Human Inner Cell Mass During Embryonic Stem Cell Derivation

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2012 Feb 29

PMID 22371082

Citations 60

Authors

Thomas OLeary

Bjorn Heindryckx

Sylvie Lierman

David van Bruggen

Jelle J Goeman

Mado Vandewoestyne

Dieter Deforce

Susana M Chuva de Sousa Lopes

Petra De Sutter

Affiliations

Soon will be listed here.

Abstract

The different pluripotent states of mouse embryonic stem cells (ESCs) in vitro have been shown to correspond to stages of mouse embryonic development. For human cells, little is known about the events that precede the generation of ESCs or whether they correlate with in vivo developmental stages. Here we investigate the cellular and molecular changes that occur during the transition from the human inner cell mass (ICM) to ESCs in vitro. We demonstrate that human ESCs originate from a post-ICM intermediate (PICMI), a transient epiblast-like structure that has undergone X-inactivation in female cells and is both necessary and sufficient for ESC derivation. The PICMI is the result of progressive and defined ICM organization in vitro and has a distinct state of cell signaling. The PICMI can be cryopreserved without compromising ESC derivation capacity. As a closer progenitor of ESCs than the ICM, the PICMI provides insight into the pluripotent state of human stem cells.

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