» Articles » PMID: 22270015

Differential Proteome and Transcriptome Analysis of Porcine Skeletal Muscle During Development

Overview
Journal J Proteomics
Publisher Elsevier
Specialty Biochemistry
Date 2012 Jan 25
PMID 22270015
Citations 26
Authors
Affiliations
Soon will be listed here.
Abstract

To gain further insight into the molecular mechanism of porcine skeletal muscle development, we combined MS characterization of proteins with high-throughput screening of differential mRNAs obtained from purebred Meishan longissimus dorsi muscle (LM) at four stages of 65 days post conception, 3, 60 and 120 days after birth. Strikingly, the dramatic differences were observed in embryo and newborn pigs, whereas 60 and 120 days pigs exhibited similar patterns in protein and mRNA expression. At the protein level, 66 differentially expressed proteins were identified. The development-dependent alterations in protein abundance indicated dramatic changes in metabolism, myofibrillar filaments, cytoskeleton, contractile activity and stress response, and signal transduction. At the transcript level, gene expression was measured with the Affymetrix Porcine Genechip, and 338 genes, representing approximately 1.7% of the chromosome, differed by two fold or more between the neighboring growth phases. Analysis of one such comparison, the expression patterns of most differential proteins showed a positive correlation with their gene expression at the transcript level during skeletal muscle development. Overall, many proteins or genes were previously unrecognized as differentially expressed during growth stages, and they represented novel starting points for understanding the developmental characteristics of biochemical and physiological properties in porcine skeletal muscle.

Citing Articles

EGR1 mRNA expression levels and polymorphisms are associated with slaughter performance in chickens.

Ye M, Chao X, Ye C, Guo L, Fan Z, Ma X Poult Sci. 2024; 104(1):104533.

PMID: 39603185 PMC: 11635649. DOI: 10.1016/j.psj.2024.104533.


Heat Stress Induces Alterations in Gene Expression of Actin Cytoskeleton and Filament of Cellular Components Causing Gut Disruption in Growing-Finishing Pigs.

Choi Y, Park H, Kim J, Lee H, Kim M Animals (Basel). 2024; 14(17).

PMID: 39272260 PMC: 11394201. DOI: 10.3390/ani14172476.


Mass Spectrometry-Based Proteomic Technology and Its Application to Study Skeletal Muscle Cell Biology.

Dowling P, Swandulla D, Ohlendieck K Cells. 2023; 12(21).

PMID: 37947638 PMC: 10649384. DOI: 10.3390/cells12212560.


Whole-genome DNA methylation profiling reveals epigenetic signatures in developing muscle in Tan and Hu sheep and their offspring.

Yue C, Wang J, Shen Y, Zhang J, Liu J, Xiao A Front Vet Sci. 2023; 10:1186040.

PMID: 37388464 PMC: 10301830. DOI: 10.3389/fvets.2023.1186040.


Transcriptome profile of spleen tissues from locally-adapted Kenyan pigs (Sus scrofa) experimentally infected with three varying doses of a highly virulent African swine fever virus genotype IX isolate: Ken12/busia.1 (ken-1033).

Machuka E, Juma J, Muigai A, Amimo J, Pelle R, Abworo E BMC Genomics. 2022; 23(1):522.

PMID: 35854219 PMC: 9294756. DOI: 10.1186/s12864-022-08754-8.