The Expression Pattern of in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Oct 23

PMID 31635221

Citations 4

Authors

Jianyu Ma

Caifang Ren

Hua Yang

Jie Zhao

Feng Wang

Yongjie Wan

Affiliations

Soon will be listed here.

Abstract

The complement 1q binding protein C (), also known as , is highly expressed in rapidly growing tissues and plays a crucial role in cell proliferation and apoptosis. However, there are no data interpreting its mechanisms in muscle development. To investigate the role of in sheep muscle development, an 856 bp cDNA fragment of containing an 837 bp coding sequence that encodes 278 amino acids was analyzed. We then revealed that the expression of in the longissimus and quadricep muscles of fetal sheep was more significantly up-regulated than expression at other developmental stages. Furthermore, we found that the expression of was increased during myoblasts differentiation in vitro. Additionally, the knockdown of in sheep myoblasts effectively inhibited myoblast differentiation, proliferation, and promoted cell apoptosis in vitro. The interference of also changed the energy metabolism from Oxidative Phosphorylation (OXPHOS) to glycolysis and activated AMP-activated protein kinase () phosphorylation in sheep myoblasts in vitro. Taken together, our data suggest that plays a vital role in the development of sheep muscle and provides a potential direction for future research on muscle development and some muscle diseases.

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