Selection of Candidate Genes Affecting Meat Quality and Preliminary Exploration of Related Molecular Mechanisms in the Mashen Pig

Overview

Journal Asian-Australas J Anim Sci

Specialty Veterinary Medicine

Date 2019 Apr 24

PMID 31010998

Citations 10

Authors

Pengfei Gao

Zhimin Cheng

Meng Li

Ningfang Zhang

Baoyu Le

Wanfeng Zhang

Pengkang Song

Xiaohong Guo

Bugao Li

Guoqing Cao

Affiliations

Soon will be listed here.

Abstract

Objective: The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig.

Methods: The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs).

Results: The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways.

Conclusion: Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs.

Citing Articles

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