Neuronostatin, a Novel Peptide Encoded by Somatostatin Gene, Regulates Cardiac Contractile Function and Cardiomyocyte Survival

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2011 Dec 16

PMID 22170057

Citations 7

Authors

Laura Vainio

Abel Perjes

Niilo Ryti

Johanna Magga

Tarja Alakoski

Raisa Serpi

Leena Kaikkonen

Jarkko Piuhola

Istvan Szokodi

Heikki Ruskoaho

Risto Kerkela

Affiliations

Soon will be listed here.

Abstract

Neuronostatin, a recently discovered peptide encoded by somatostatin gene, is involved in regulation of neuronal function, blood pressure, food intake, and drinking behavior. However, the biological effects of neuronostatin on cardiac myocytes are not known, and the intracellular signaling mechanisms induced by neuronostatin remain unidentified. We analyzed the effect of neuronostatin in isolated perfused rat hearts and in cultured primary cardiomyocytes. Neuronostatin infusion alone had no effect on left ventricular (LV) contractile function or on isoprenaline- or preload-induced increase in cardiac contractility. However, infusion of neuronostatin significantly decreased the positive inotropic response to endothelin-1 (ET-1). This was associated with an increase in phosphorylation of p38 mitogen-activated protein kinase and c-Jun N-terminal kinase (JNK). Treatment of both neonatal and adult cardiomyocytes with neuronostatin resulted in reduced cardiomyocyte viability. Inhibition of JNK further increased the neuronostatin-induced cell death. We conclude that neuronostatin regulates cardiac contractile function and cardiomyocyte survival. Receptors for neuronostatin need to be identified to further characterize the biological functions of the peptide.

Citing Articles

Regulatory Mechanisms of Somatostatin Expression.

Ampofo E, Nalbach L, Menger M, Laschke M Int J Mol Sci. 2020; 21(11).

PMID: 32545257 PMC: 7312888. DOI: 10.3390/ijms21114170.

Conceptualization of a Parasympathetic Endocrine System.

Gorky J, Schwaber J Front Neurosci. 2019; 13:1008.

PMID: 31607849 PMC: 6767939. DOI: 10.3389/fnins.2019.01008.

Association between a polymorphic poly-T repeat sequence in the promoter of the somatostatin gene and hypertension.

Tremblay M, Brisson D, Gaudet D Hypertens Res. 2016; 39(6):467-74.

PMID: 26818653 DOI: 10.1038/hr.2016.4.

Understanding peptide biology: The discovery and characterization of the novel hormone, neuronostatin.

Yosten G, Elrick M, Salvatori A, Stein L, Kolar G, Ren J Peptides. 2015; 72:192-5.

PMID: 26051024 PMC: 4641813. DOI: 10.1016/j.peptides.2015.05.011.

Isolation and physiological analysis of mouse cardiomyocytes.

Roth G, Bader D, Pfaltzgraff E J Vis Exp. 2014; (91):e51109.

PMID: 25225886 PMC: 4828048. DOI: 10.3791/51109.

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