Genetic and Sequence Analysis of an 8.7-kilobase Pseudomonas Denitrificans Fragment Carrying Eight Genes Involved in Transformation of Precorrin-2 to Cobyrinic Acid

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 Oct 1

PMID 2211521

Citations 29

Authors

J Crouzet

B Cameron

L Cauchois

S Rigault

M C Rouyez

F Blanche

D THIBAUT

L Debussche

Affiliations

Soon will be listed here.

Abstract

A 8.7-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and the genetic analysis revealed that this fragment carries eight different cob genes (cobF to cobM). Six of these genes have the characteristics of translationally coupled genes. cobI has been identified as S-adenosyl-L-methionine (SAM):precorrin-2 methyltransferase structural gene because the encoded protein has the same NH2 terminus and molecular weight as those of the purified enzyme. From protein homology with CobA and CobI, two SAM-dependent methyltransferases of the cobalamin pathway, it is proposed that cobF, cobJ, cobL, and cobM code for other methyltransferases involved in the cobalamin pathway. In addition, purified CobF protein has affinity for SAM, as expected for a SAM-dependent methyltransferase. Accumulation of cobalamin precursors in Agrobacterium tumefaciens mutants complemented by any of these eight genes suggest that, apart from cobI, whose function is identified, the products of all these genes are implicated in the conversion of precorrin-3 into cobyrinic acid.

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References

Boyer H . A complementation analysis of the restriction and modification of DNA in Escherichia coli. J Mol Biol. 1969; 41(3):459-72. DOI: 10.1016/0022-2836(69)90288-5. View

Aksoy S, Squires C, Squires C . Translational coupling of the trpB and trpA genes in the Escherichia coli tryptophan operon. J Bacteriol. 1984; 157(2):363-7. PMC: 215255. DOI: 10.1128/jb.157.2.363-367.1984. View

Figurski D, Helinski D . Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc Natl Acad Sci U S A. 1979; 76(4):1648-52. PMC: 383447. DOI: 10.1073/pnas.76.4.1648. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Hopp T, Woods K . Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981; 78(6):3824-8. PMC: 319665. DOI: 10.1073/pnas.78.6.3824. View

Staden R, McLachlan A . Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Res. 1982; 10(1):141-56. PMC: 326122. DOI: 10.1093/nar/10.1.141. View

Brey R, Banner C, Wolf J . Cloning of multiple genes involved with cobalamin (Vitamin B12) biosynthesis in Bacillus megaterium. J Bacteriol. 1986; 167(2):623-30. PMC: 212935. DOI: 10.1128/jb.167.2.623-630.1986. View

Platt T . Transcription termination and the regulation of gene expression. Annu Rev Biochem. 1986; 55:339-72. DOI: 10.1146/annurev.bi.55.070186.002011. View

Ben-Bassat A, Bauer K, Chang S, Myambo K, Boosman A, Chang S . Processing of the initiation methionine from proteins: properties of the Escherichia coli methionine aminopeptidase and its gene structure. J Bacteriol. 1987; 169(2):751-7. PMC: 211843. DOI: 10.1128/jb.169.2.751-757.1987. View

10.

Jeter R, Roth J . Cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium. J Bacteriol. 1987; 169(7):3189-98. PMC: 212369. DOI: 10.1128/jb.169.7.3189-3198.1987. View

11.

West S, Iglewski B . Codon usage in Pseudomonas aeruginosa. Nucleic Acids Res. 1988; 16(19):9323-35. PMC: 338709. DOI: 10.1093/nar/16.19.9323. View

12.

Cameron B, Briggs K, Pridmore S, Brefort G, Crouzet J . Cloning and analysis of genes involved in coenzyme B12 biosynthesis in Pseudomonas denitrificans. J Bacteriol. 1989; 171(1):547-57. PMC: 209620. DOI: 10.1128/jb.171.1.547-557.1989. View

13.

Posfai J, Bhagwat A, Posfai G, Roberts R . Predictive motifs derived from cytosine methyltransferases. Nucleic Acids Res. 1989; 17(7):2421-35. PMC: 317633. DOI: 10.1093/nar/17.7.2421. View

14.

Leeper F . The biosynthesis of porphyrins, chlorophylls, and vitamin B12. Nat Prod Rep. 1989; 6(2):171-203. DOI: 10.1039/np9890600171. View

15.

Blanche F, Debussche L, THIBAUT D, Crouzet J, Cameron B . Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans. J Bacteriol. 1989; 171(8):4222-31. PMC: 210194. DOI: 10.1128/jb.171.8.4222-4231.1989. View

16.

Hirel P, Schmitter M, Dessen P, Fayat G, Blanquet S . Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Proc Natl Acad Sci U S A. 1989; 86(21):8247-51. PMC: 298257. DOI: 10.1073/pnas.86.21.8247. View

17.

Crouzet J, Cauchois L, Blanche F, Debussche L, THIBAUT D, Rouyez M . Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide.... J Bacteriol. 1990; 172(10):5968-79. PMC: 526919. DOI: 10.1128/jb.172.10.5968-5979.1990. View

18.

Kanehisa M . Use of statistical criteria for screening potential homologies in nucleic acid sequences. Nucleic Acids Res. 1984; 12(1 Pt 1):203-13. PMC: 320997. DOI: 10.1093/nar/12.1part1.203. View

19.

Staden R . Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Res. 1984; 12(1 Pt 2):551-67. PMC: 321070. DOI: 10.1093/nar/12.1part2.551. View

20.

Normark S, Bergstrom S, Edlund T, Grundstrom T, Jaurin B, Lindberg F . Overlapping genes. Annu Rev Genet. 1983; 17:499-525. DOI: 10.1146/annurev.ge.17.120183.002435. View