Cobalamin (vitamin B12) Biosynthetic Genes of Salmonella Typhimurium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1987 Jul 1

PMID 3036774

Citations 36

Authors

R M Jeter

J R Roth

Affiliations

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Abstract

The enteric bacterium Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo only under anaerobic growth conditions. We initiated a genetic analysis of the cobalamin biosynthetic (cob) gene cluster, Stable cob::lac operon fusions were generated by insertions of a transposition-defective derivative of bacteriophage Mu d1 (Ap lac) into the cob genes. beta-Galactosidase synthesis was repressed in the presence of exogenously supplied cyanocobalamin, demonstrating that the cobalamin biosynthetic pathway was regulated by end-product repression. Transcriptional polarity studies showed that the cob genes responsible for synthesis of the corrinoid intermediate cobinamide (branch I of the pathway) were organized into a single operon. Genes for the synthesis of 5,6-dimethylbenzimidazole (branch II) and the final assembly of the complete cobalamin molecule (branch III) were organized into two or more additional operons. All of the known cob genes (in branches I, II, and III) were transcribed in a counterclockwise direction relative to the S. typhimurium genetic map. These genes are located at 41 map units and near the his operon. No essential genes lie between the his and cob operons. Mutants that carried deletions extending from the his genes into the cob region were isolated and characterized. By using these mutants, a deletion map of the branch I cob operon was constructed and the order of genes (his-cobI-cobIII-cobII) was inferred.

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References

Hartman P, Rusgis C, STAHL R . Orientation of the histidine operon in the Salmonella typhimurium linkage map. Proc Natl Acad Sci U S A. 1965; 53(6):1332-5. PMC: 219847. DOI: 10.1073/pnas.53.6.1332. View

Vogel H, Bonner D . Acetylornithinase of Escherichia coli: partial purification and some properties. J Biol Chem. 1956; 218(1):97-106. View

Smith H, Levine M . A phage P22 gene controlling integration of prophage. Virology. 1967; 31(2):207-16. DOI: 10.1016/0042-6822(67)90164-x. View

Fink G, Klopotowski T, Ames B . Histidine regulatory mutants in Salmonella typhimurium. IV. A positive selection for polar histidine-requiring mutants from histidine operator constitutive mutants. J Mol Biol. 1967; 30(1):81-95. DOI: 10.1016/0022-2836(67)90245-8. View

Childs J, Smith D . New methionine structural gene in Salmonella typhimurium. J Bacteriol. 1969; 100(1):377-82. PMC: 315403. DOI: 10.1128/jb.100.1.377-382.1969. View

Chang G, Chang J . Evidence for the B12-dependent enzyme ethanolamine deaminase in Salmonella. Nature. 1975; 254(5496):150-1. DOI: 10.1038/254150a0. View

Kleckner N, Roth J, Botstein D . Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977; 116(1):125-59. DOI: 10.1016/0022-2836(77)90123-1. View

Anderson R, Roth J . Tandem chromosomal duplications in Salmonella typhimurium: fusion of histidine genes to novel promoters. J Mol Biol. 1978; 119(1):147-66. DOI: 10.1016/0022-2836(78)90274-7. View

Kleckner N, Reichardt K, Botstein D . Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10. J Mol Biol. 1979; 127(1):89-115. DOI: 10.1016/0022-2836(79)90461-3. View

10.

Casadaban M, Cohen S . Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979; 76(9):4530-3. PMC: 411611. DOI: 10.1073/pnas.76.9.4530. View

11.

Balch W, Fox G, Magrum L, Woese C, WOLFE R . Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979; 43(2):260-96. PMC: 281474. DOI: 10.1128/mr.43.2.260-296.1979. View

12.

Johnston H, Barnes W, Chumley F, Bossi L, Roth J . Model for regulation of the histidine operon of Salmonella. Proc Natl Acad Sci U S A. 1980; 77(1):508-12. PMC: 348301. DOI: 10.1073/pnas.77.1.508. View

13.

Maloy S, Nunn W . Selection for loss of tetracycline resistance by Escherichia coli. J Bacteriol. 1981; 145(2):1110-1. PMC: 217228. DOI: 10.1128/jb.145.2.1110-1111.1981. View

14.

Bochner B, Huang H, Schieven G, Ames B . Positive selection for loss of tetracycline resistance. J Bacteriol. 1980; 143(2):926-33. PMC: 294396. DOI: 10.1128/jb.143.2.926-933.1980. View

15.

Anderson P, Roth J . Spontaneous tandem genetic duplications in Salmonella typhimurium arise by unequal recombination between rRNA (rrn) cistrons. Proc Natl Acad Sci U S A. 1981; 78(5):3113-7. PMC: 319510. DOI: 10.1073/pnas.78.5.3113. View

16.

Ciampi M, Schmid M, Roth J . Transposon Tn10 provides a promoter for transcription of adjacent sequences. Proc Natl Acad Sci U S A. 1982; 79(16):5016-20. PMC: 346817. DOI: 10.1073/pnas.79.16.5016. View

17.

Simons R, Hoopes B, McClure W, Kleckner N . Three promoters near the termini of IS10: pIN, pOUT, and pIII. Cell. 1983; 34(2):673-82. DOI: 10.1016/0092-8674(83)90400-2. View

18.

Sanderson K, Roth J . Linkage map of Salmonella typhimurium, Edition VI. Microbiol Rev. 1983; 47(3):410-53. PMC: 281582. DOI: 10.1128/mr.47.3.410-453.1983. View

19.

Hughes K, Roth J . Conditionally transposition-defective derivative of Mu d1(Amp Lac). J Bacteriol. 1984; 159(1):130-7. PMC: 215603. DOI: 10.1128/jb.159.1.130-137.1984. View

20.

Jeter R, Olivera B, Roth J . Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic growth conditions. J Bacteriol. 1984; 159(1):206-13. PMC: 215614. DOI: 10.1128/jb.159.1.206-213.1984. View