Genetic and Sequence Analyses of a Pseudomonas Denitrificans DNA Fragment Containing Two Cob Genes

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Oct 1

PMID 1917840

Citations 15

Authors

B Cameron

C Guilhot

F Blanche

L Cauchois

M C Rouyez

S Rigault

J Crouzet

Affiliations

Soon will be listed here.

Abstract

A genetic analysis of a 12-kb DNA fragment containing Pseudomonas denitrificans cob genes was performed by transposon-mediated insertional mutagenesis. The nucleotide sequence and genetic analysis have shown that a 4.8-kb DNA subfragment carried two cob genes (cobS and cobT). Biochemical data concerning the complemented cobS and cobT mutants suggested that the cobS product was involved in cobalt insertion-mediating reactions and that the cobT product was involved in the transformation of precorrin-3 into cobyrinic acid.

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