Nucleotide Sequence of a Pseudomonas Denitrificans 5.4-kilobase DNA Fragment Containing Five Cob Genes and Identification of Structural Genes Encoding S-adenosyl-L-methionine: Uroporphyrinogen III Methyltransferase and Cobyrinic Acid A,c-diamide...

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1990 Oct 1

PMID 2211520

Citations 36

Authors

J Crouzet

L Cauchois

F Blanche

L Debussche

D THIBAUT

M C Rouyez

S Rigault

J F Mayaux

B Cameron

Affiliations

Soon will be listed here.

Abstract

A 5.4-kilobase DNA fragment carrying Pseudomonas denitrificans cob genes has been sequenced. The nucleotide sequence and genetic analysis revealed that this fragment carries five different cob genes (cobA to cobE). Four of these genes present the characteristics of translationally coupled genes. cobA has been identified as the structural gene of S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) because the encoded protein has the same NH2 terminus and molecular weight as those determined for the purified SUMT. For the same reasons the cobB gene was shown to be the structural gene for cobyrinic acid a,c-diamide synthase. Genetic and biochemical data concerning cobC and cobD mutants suggest that the products of these genes are involved in the conversion of cobyric acid to cobinamide.

Citing Articles

Bioprocess Strategies for Vitamin B Production by Microbial Fermentation and Its Market Applications.

Calvillo A, Pellicer T, Carnicer M, Planas A Bioengineering (Basel). 2022; 9(8).

PMID: 36004890 PMC: 9405231. DOI: 10.3390/bioengineering9080365.

Construction of Fluorescent Analogs to Follow the Uptake and Distribution of Cobalamin (Vitamin B) in Bacteria, Worms, and Plants.

Lawrence A, Nemoto-Smith E, Deery E, Baker J, Schroeder S, Brown D Cell Chem Biol. 2018; 25(8):941-951.e6.

PMID: 29779954 PMC: 6125784. DOI: 10.1016/j.chembiol.2018.04.012.

Sulfite reduction in mycobacteria.

Pinto R, Harrison J, Hsu T, Jacobs Jr W, Leyh T J Bacteriol. 2007; 189(18):6714-22.

PMID: 17644602 PMC: 2045171. DOI: 10.1128/JB.00487-07.

Identification and functional analysis of enzymes required for precorrin-2 dehydrogenation and metal ion insertion in the biosynthesis of sirohaem and cobalamin in Bacillus megaterium.

Raux E, Leech H, Beck R, Schubert H, Santander P, Roessner C Biochem J. 2002; 370(Pt 2):505-16.

PMID: 12408752 PMC: 1223173. DOI: 10.1042/BJ20021443.

Cell biology and molecular basis of denitrification.

Zumft W Microbiol Mol Biol Rev. 1997; 61(4):533-616.

PMID: 9409151 PMC: 232623. DOI: 10.1128/mmbr.61.4.533-616.1997.

References

Shine J, Dalgarno L . The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974; 71(4):1342-6. PMC: 388224. DOI: 10.1073/pnas.71.4.1342. View

Andersson D, Roth J . Mutations affecting regulation of cobinamide biosynthesis in Salmonella typhimurium. J Bacteriol. 1989; 171(12):6726-33. PMC: 210569. DOI: 10.1128/jb.171.12.6726-6733.1989. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Granick S, Beale S . Hemes, chlorophylls, and related compounds: biosynthesis and metabolic regulation. Adv Enzymol Relat Areas Mol Biol. 1978; 46:33-203. DOI: 10.1002/9780470122914.ch2. View

Casadaban M, Cohen S . Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc Natl Acad Sci U S A. 1979; 76(9):4530-3. PMC: 411611. DOI: 10.1073/pnas.76.9.4530. View

BATTERSBY A, Fookes C, Matcham G, McDonald E . Biosynthesis of the pigments of life: formation of the macrocycle. Nature. 1980; 285(5759):17-21. DOI: 10.1038/285017a0. View

Ditta G, Stanfield S, Corbin D, Helinski D . Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci U S A. 1980; 77(12):7347-51. PMC: 350500. DOI: 10.1073/pnas.77.12.7347. View

Staden R, McLachlan A . Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Res. 1982; 10(1):141-56. PMC: 326122. DOI: 10.1093/nar/10.1.141. View

Cossart P . Cloning and sequence of the crp gene of Escherichia coli K 12. Nucleic Acids Res. 1982; 10(4):1363-78. PMC: 320531. DOI: 10.1093/nar/10.4.1363. View

10.

Bagdasarian M, Lurz R, Ruckert B, Franklin F, Bagdasarian M, Frey J . Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors, and a host-vector system for gene cloning in Pseudomonas. Gene. 1981; 16(1-3):237-47. DOI: 10.1016/0378-1119(81)90080-9. View

11.

Grosjean H, Fiers W . Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes. Gene. 1982; 18(3):199-209. DOI: 10.1016/0378-1119(82)90157-3. View

12.

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

13.

Casadaban M, Martinez-Arias A, Shapira S, Chou J . Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol. 1983; 100:293-308. DOI: 10.1016/0076-6879(83)00063-4. View

14.

Kanehisa M . Use of statistical criteria for screening potential homologies in nucleic acid sequences. Nucleic Acids Res. 1984; 12(1 Pt 1):203-13. PMC: 320997. DOI: 10.1093/nar/12.1part1.203. View

15.

Staden R . Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Res. 1984; 12(1 Pt 2):551-67. PMC: 321070. DOI: 10.1093/nar/12.1part2.551. View

16.

Normark S, Bergstrom S, Edlund T, Grundstrom T, Jaurin B, Lindberg F . Overlapping genes. Annu Rev Genet. 1983; 17:499-525. DOI: 10.1146/annurev.ge.17.120183.002435. View

17.

Norrander J, Kempe T, Messing J . Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene. 1983; 26(1):101-6. DOI: 10.1016/0378-1119(83)90040-9. View

18.

Kieny M, Lathe R, Lecocq J . New versatile cloning and sequencing vectors based on bacteriophage M13. Gene. 1983; 26(1):91-9. DOI: 10.1016/0378-1119(83)90039-2. View

19.

de Bruijn F, Lupski J . The use of transposon Tn5 mutagenesis in the rapid generation of correlated physical and genetic maps of DNA segments cloned into multicopy plasmids--a review. Gene. 1984; 27(2):131-49. DOI: 10.1016/0378-1119(84)90135-5. View

20.

Jeter R, Olivera B, Roth J . Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic growth conditions. J Bacteriol. 1984; 159(1):206-13. PMC: 215614. DOI: 10.1128/jb.159.1.206-213.1984. View