Human Pluripotent Stem Cell-based Approaches for Myocardial Repair: from the Electrophysiological Perspective

Overview

Journal Mol Pharm

Publisher American Chemical Society

Specialty Pharmacology

Date 2011 Sep 2

PMID 21879736

Citations 28

Authors

Ellen Poon

Chi-Wing Kong

Ronald A Li

Affiliations

Soon will be listed here.

Abstract

Heart diseases are a leading cause of mortality worldwide. Terminally differentiated adult cardiomyocytes (CMs) lack the innate ability to regenerate. Their malfunction or significant loss can lead to conditions from cardiac arrhythmias to heart failure. For myocardial repair, cell- and gene-based therapies offer promising alternatives to donor organ transplantation. Human embryonic stem cells (hESCs) can self-renew while maintaining their pluripotency. Direct reprogramming of adult somatic cells to become pluripotent hES-like cells (also known as induced pluripotent stem cells or iPSCs) has been achieved. Both hESCs and iPSCs have been successfully differentiated into genuine human CMs. In this review, we describe our current knowledge of the structure-function properties of hESC/iPSC-CMs, with an emphasis on their electrophysiology and Ca(2+) handling, along with the hurdles faced and potential solutions for translating into clinical and other applications (e.g., disease modeling, cardiotoxicity and drug screening).

Citing Articles

Membrane Remodeling of Human-Engineered Cardiac Tissue by Chronic Electric Stimulation.

Sesena-Rubfiaro A, Prajapati N, Paolino L, Lou L, Cotayo D, Pandey P ACS Biomater Sci Eng. 2023; 9(3):1644-1655.

PMID: 36765460 PMC: 10542861. DOI: 10.1021/acsbiomaterials.2c01370.

Stimulating Calcium Handling in hiPSC-Derived Engineered Cardiac Tissues Enhances Force Production.

Minor A, Coulombe K Stem Cells Transl Med. 2022; 11(1):97-106.

PMID: 35641165 PMC: 8895492. DOI: 10.1093/stcltm/szab002.

Cell surface markers for immunophenotyping human pluripotent stem cell-derived cardiomyocytes.

Boheler K, Poon E Pflugers Arch. 2021; 473(7):1023-1039.

PMID: 33928456 DOI: 10.1007/s00424-021-02549-8.

Microscale grooves regulate maturation development of hPSC-CMs by the transient receptor potential channels (TRP channels).

Liu T, Zhang S, Huang C, Ma S, Bai R, Li Y J Cell Mol Med. 2021; 25(7):3469-3483.

PMID: 33689230 PMC: 8034460. DOI: 10.1111/jcmm.16429.

Age-Dependent Maturation of iPSC-CMs Leads to the Enhanced Compartmentation of βAR-cAMP Signalling.

Hasan A, Mohammadi N, Nawaz A, Kodagoda T, Diakonov I, Harding S Cells. 2020; 9(10).

PMID: 33053822 PMC: 7601768. DOI: 10.3390/cells9102275.

References

Bu L, Jiang X, Martin-Puig S, Caron L, Zhu S, Shao Y . Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages. Nature. 2009; 460(7251):113-7. DOI: 10.1038/nature08191. View

Fu J, Jiang P, Rushing S, Liu J, Chiamvimonvat N, Li R . Na+/Ca2+ exchanger is a determinant of excitation-contraction coupling in human embryonic stem cell-derived ventricular cardiomyocytes. Stem Cells Dev. 2009; 19(6):773-82. PMC: 3135244. DOI: 10.1089/scd.2009.0184. View

Huangfu D, Osafune K, Maehr R, Guo W, Eijkelenboom A, Chen S . Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol. 2008; 26(11):1269-75. DOI: 10.1038/nbt.1502. View

Ambros V . The functions of animal microRNAs. Nature. 2004; 431(7006):350-5. DOI: 10.1038/nature02871. View

Boheler K, Czyz J, Tweedie D, Yang H, Anisimov S, Wobus A . Differentiation of pluripotent embryonic stem cells into cardiomyocytes. Circ Res. 2002; 91(3):189-201. DOI: 10.1161/01.res.0000027865.61704.32. View

Xue T, Cho H, Akar F, Tsang S, Jones S, Marban E . Functional integration of electrically active cardiac derivatives from genetically engineered human embryonic stem cells with quiescent recipient ventricular cardiomyocytes: insights into the development of cell-based pacemakers. Circulation. 2004; 111(1):11-20. DOI: 10.1161/01.CIR.0000151313.18547.A2. View

Tse H, Xue T, Lau C, Siu C, Wang K, Zhang Q . Bioartificial sinus node constructed via in vivo gene transfer of an engineered pacemaker HCN Channel reduces the dependence on electronic pacemaker in a sick-sinus syndrome model. Circulation. 2006; 114(10):1000-11. DOI: 10.1161/CIRCULATIONAHA.106.615385. View

Moore J, Fu J, Chan Y, Lin D, Tran H, Tse H . Distinct cardiogenic preferences of two human embryonic stem cell (hESC) lines are imprinted in their proteomes in the pluripotent state. Biochem Biophys Res Commun. 2008; 372(4):553-8. PMC: 2665880. DOI: 10.1016/j.bbrc.2008.05.076. View

Yang L, Soonpaa M, Adler E, Roepke T, Kattman S, Kennedy M . Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. Nature. 2008; 453(7194):524-8. DOI: 10.1038/nature06894. View

10.

Wilson K, Hu S, Venkatasubrahmanyam S, Fu J, Sun N, Abilez O . Dynamic microRNA expression programs during cardiac differentiation of human embryonic stem cells: role for miR-499. Circ Cardiovasc Genet. 2010; 3(5):426-35. PMC: 3057038. DOI: 10.1161/CIRCGENETICS.109.934281. View

11.

Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T . Identification of tissue-specific microRNAs from mouse. Curr Biol. 2002; 12(9):735-9. DOI: 10.1016/s0960-9822(02)00809-6. View

12.

He J, Ma Y, Lee Y, Thomson J, Kamp T . Human embryonic stem cells develop into multiple types of cardiac myocytes: action potential characterization. Circ Res. 2003; 93(1):32-9. DOI: 10.1161/01.RES.0000080317.92718.99. View

13.

Buckingham M, Meilhac S, Zaffran S . Building the mammalian heart from two sources of myocardial cells. Nat Rev Genet. 2005; 6(11):826-35. DOI: 10.1038/nrg1710. View

14.

Zwi L, Caspi O, Arbel G, Huber I, Gepstein A, Park I . Cardiomyocyte differentiation of human induced pluripotent stem cells. Circulation. 2009; 120(15):1513-23. DOI: 10.1161/CIRCULATIONAHA.109.868885. View

15.

Brette F, Orchard C . Resurgence of cardiac t-tubule research. Physiology (Bethesda). 2007; 22:167-73. DOI: 10.1152/physiol.00005.2007. View

16.

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

17.

Passier R, Ward-van Oostwaard D, Snapper J, Kloots J, Hassink R, Kuijk E . Increased cardiomyocyte differentiation from human embryonic stem cells in serum-free cultures. Stem Cells. 2005; 23(6):772-80. DOI: 10.1634/stemcells.2004-0184. View

18.

McGill C, Brooks G . Cell cycle control mechanisms and their role in cardiac growth. Cardiovasc Res. 1995; 30(4):557-69. View

19.

Balsam L, Wagers A, Christensen J, Kofidis T, Weissman I, Robbins R . Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium. Nature. 2004; 428(6983):668-73. DOI: 10.1038/nature02460. View

20.

Eschenhagen T, Zimmermann W . Engineering myocardial tissue. Circ Res. 2005; 97(12):1220-31. DOI: 10.1161/01.RES.0000196562.73231.7d. View