C-Jun N-terminal Kinase Phosphorylates DCP1a to Control Formation of P Bodies

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2011 Aug 24

PMID 21859862

Citations 53

Authors

Katharina Rzeczkowski

Knut Beuerlein

Helmut Muller

Oliver Dittrich-Breiholz

Heike Schneider

Daniela Kettner-Buhrow

Helmut Holtmann

Michael Kracht

Affiliations

Soon will be listed here.

Abstract

Cytokines and stress-inducing stimuli signal through c-Jun N-terminal kinase (JNK) using a diverse and only partially defined set of downstream effectors. In this paper, the decapping complex subunit DCP1a was identified as a novel JNK target. JNK phosphorylated DCP1a at residue S315 in vivo and in vitro and coimmunoprecipitated and colocalized with DCP1a in processing bodies (P bodies). Sustained JNK activation by several different inducers led to DCP1a dispersion from P bodies, whereas IL-1 treatment transiently increased P body number. Inhibition of TAK1-JNK signaling also affected the number and size of P bodies and the localization of DCP1a, Xrn1, and Edc4. Transcriptome analysis further identified a central role for DCP1a in IL-1-induced messenger ribonucleic acid (mRNA) expression. Phosphomimetic mutation of S315 stabilized IL-8 but not IκBα mRNA, whereas overexpressed DCP1a blocked IL-8 transcription and suppressed p65 NF-κB nuclear activity. Collectively, these data reveal DCP1a as a multifunctional regulator of mRNA expression and suggest a novel mechanism controlling the subcellular localization of DCP1a in response to stress or inflammatory stimuli.

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