Eukaryotic Stress Granules: the Ins and Outs of Translation

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2010 Jan 13

PMID 20064460

Citations 781

Authors

J Ross Buchan

Roy Parker

Affiliations

Soon will be listed here.

Abstract

The stress response in eukaryotic cells often inhibits translation initiation and leads to the formation of cytoplasmic RNA-protein complexes referred to as stress granules. Stress granules contain nontranslating mRNAs, translation initiation components, and many additional proteins affecting mRNA function. Stress granules have been proposed to affect mRNA translation and stability and have been linked to apoptosis and nuclear processes. Stress granules also interact with P-bodies, another cytoplasmic RNP granule containing nontranslating mRNA, translation repressors, and some mRNA degradation machinery. Together, stress granules and P-bodies reveal a dynamic cycle of distinct biochemical and compartmentalized mRNPs in the cytosol, with implications for the control of mRNA function.

Citing Articles

Decoding subcellular RNA localization one molecule at a time.

Biayna J, Dumbovic G Genome Biol. 2025; 26(1):45.

PMID: 40033325 PMC: 11874642. DOI: 10.1186/s13059-025-03507-8.

Systems Biology of Recombinant 2G12 and 353/11 mAb Production in CHO-K1 Cell Lines at Phosphoproteome Level.

Sulaj E, Sandell F, Schwaigerlehner L, Marzban G, Dohm J, Kunert R Proteomes. 2025; 13(1).

PMID: 39982319 PMC: 11843875. DOI: 10.3390/proteomes13010009.

Structural, biological, and biomedical implications of mRNA interactions with the master regulator HuR.

Clark M, Farinha A, Morrison A, Lisi G NAR Mol Med. 2025; 2(1):ugaf002.

PMID: 39980665 PMC: 11838611. DOI: 10.1093/narmme/ugaf002.

The zinc finger protein ZFP36L2 inhibits flavivirus infection via the 5'-3' XRN1-mediated RNA decay pathway in the replication complexes.

Lin R, Lin L, Chen Z, Liu B, Ko P, Liao C J Biomed Sci. 2025; 32(1):27.

PMID: 39972499 PMC: 11841009. DOI: 10.1186/s12929-025-01122-0.

7SL RNA and signal recognition particle orchestrate a global cellular response to acute thermal stress.

Bujisic B, Lee H, Xu L, Weissbein U, Rivera C, Topisirovic I Nat Commun. 2025; 16(1):1630.

PMID: 39952919 PMC: 11828898. DOI: 10.1038/s41467-025-56351-6.

References

Holmes L, Campbell S, De Long S, Sachs A, Ashe M . Loss of translational control in yeast compromised for the major mRNA decay pathway. Mol Cell Biol. 2004; 24(7):2998-3010. PMC: 371117. DOI: 10.1128/MCB.24.7.2998-3010.2004. View

McWhorter M, Monani U, Burghes A, Beattie C . Knockdown of the survival motor neuron (Smn) protein in zebrafish causes defects in motor axon outgrowth and pathfinding. J Cell Biol. 2003; 162(5):919-31. PMC: 1761110. DOI: 10.1083/jcb.200303168. View

Brengues M, Parker R . Accumulation of polyadenylated mRNA, Pab1p, eIF4E, and eIF4G with P-bodies in Saccharomyces cerevisiae. Mol Biol Cell. 2007; 18(7):2592-602. PMC: 1924816. DOI: 10.1091/mbc.e06-12-1149. View

Ivanov P, Chudinova E, Nadezhdina E . Disruption of microtubules inhibits cytoplasmic ribonucleoprotein stress granule formation. Exp Cell Res. 2003; 290(2):227-33. DOI: 10.1016/s0014-4827(03)00290-8. View

Kedersha N, Gupta M, Li W, Miller I, Anderson P . RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules. J Cell Biol. 1999; 147(7):1431-42. PMC: 2174242. DOI: 10.1083/jcb.147.7.1431. View

Coller J, Parker R . General translational repression by activators of mRNA decapping. Cell. 2005; 122(6):875-86. PMC: 1853273. DOI: 10.1016/j.cell.2005.07.012. View

Tourriere H, Chebli K, Zekri L, Courselaud B, Blanchard J, Bertrand E . The RasGAP-associated endoribonuclease G3BP assembles stress granules. J Cell Biol. 2003; 160(6):823-31. PMC: 2173781. DOI: 10.1083/jcb.200212128. View

Gueydan C, Droogmans L, Chalon P, Huez G, Caput D, Kruys V . Identification of TIAR as a protein binding to the translational regulatory AU-rich element of tumor necrosis factor alpha mRNA. J Biol Chem. 1999; 274(4):2322-6. DOI: 10.1074/jbc.274.4.2322. View

Tourriere H, Gallouzi I, Chebli K, Capony J, Mouaikel J, van der Geer P . RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization. Mol Cell Biol. 2001; 21(22):7747-60. PMC: 99945. DOI: 10.1128/MCB.21.22.7747-7760.2001. View

10.

Tian Q, Taupin J, Elledge S, Robertson M, Anderson P . Fas-activated serine/threonine kinase (FAST) phosphorylates TIA-1 during Fas-mediated apoptosis. J Exp Med. 1995; 182(3):865-74. PMC: 2192163. DOI: 10.1084/jem.182.3.865. View

11.

Anderson P, Kedersha N . RNA granules: post-transcriptional and epigenetic modulators of gene expression. Nat Rev Mol Cell Biol. 2009; 10(6):430-6. DOI: 10.1038/nrm2694. View

12.

van Der Houven Van Oordt W, Diaz-Meco M, Lozano J, Krainer A, Moscat J, Caceres J . The MKK(3/6)-p38-signaling cascade alters the subcellular distribution of hnRNP A1 and modulates alternative splicing regulation. J Cell Biol. 2000; 149(2):307-16. PMC: 2175157. DOI: 10.1083/jcb.149.2.307. View

13.

Aizer A, Brody Y, Ler L, Sonenberg N, Singer R, Shav-Tal Y . The dynamics of mammalian P body transport, assembly, and disassembly in vivo. Mol Biol Cell. 2008; 19(10):4154-66. PMC: 2555939. DOI: 10.1091/mbc.e08-05-0513. View

14.

Scarcelli J, Viggiano S, Hodge C, Heath C, Amberg D, Cole C . Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components. Genetics. 2008; 179(4):1945-55. PMC: 2516071. DOI: 10.1534/genetics.108.091256. View

15.

Ohn T, Kedersha N, Hickman T, Tisdale S, Anderson P . A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly. Nat Cell Biol. 2008; 10(10):1224-31. PMC: 4318256. DOI: 10.1038/ncb1783. View

16.

Gowrishankar G, Winzen R, Dittrich-Breiholz O, Redich N, Kracht M, Holtmann H . Inhibition of mRNA deadenylation and degradation by different types of cell stress. Biol Chem. 2006; 387(3):323-7. DOI: 10.1515/BC.2006.043. View

17.

Beckham C, Hilliker A, Cziko A, Noueiry A, Ramaswami M, Parker R . The DEAD-box RNA helicase Ded1p affects and accumulates in Saccharomyces cerevisiae P-bodies. Mol Biol Cell. 2007; 19(3):984-93. PMC: 2262982. DOI: 10.1091/mbc.e07-09-0954. View

18.

Mollet S, Cougot N, Wilczynska A, Dautry F, Kress M, Bertrand E . Translationally repressed mRNA transiently cycles through stress granules during stress. Mol Biol Cell. 2008; 19(10):4469-79. PMC: 2555929. DOI: 10.1091/mbc.e08-05-0499. View

19.

Martin K, Ephrussi A . mRNA localization: gene expression in the spatial dimension. Cell. 2009; 136(4):719-30. PMC: 2819924. DOI: 10.1016/j.cell.2009.01.044. View

20.

Narayanaswamy R, Levy M, Tsechansky M, Stovall G, OConnell J, Mirrielees J . Widespread reorganization of metabolic enzymes into reversible assemblies upon nutrient starvation. Proc Natl Acad Sci U S A. 2009; 106(25):10147-52. PMC: 2691686. DOI: 10.1073/pnas.0812771106. View