Deposition, Turnover, and Release of CENH3 at Arabidopsis Centromeres

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 2011 Aug 16

PMID 21842230

Citations 14

Authors

Inna Lermontova

Twan Rutten

Ingo Schubert

Affiliations

Soon will be listed here.

Abstract

The kinetochore is a complex multiprotein structure located at centromeres and required for the proper segregation of chromosomes during mitosis and meiosis. An important role in kinetochore assembly and function plays the centromeric histone H3 variant (CENH3). Cell cycle stage of CENH3 deposition to centromeres varies between different organisms. We confirmed by in vivo studies that deposition of Arabidopsis CENH3 takes place at centromeres during G2 and demonstrated that additionally a low turnover of CENH3 occurs along the cell cycle, apparently for replacement of damaged protein. Furthermore, enhanced yellow fluorescent protein (EYFP)-CENH3 of photobleached chromocenters is not replaced by EYFP-CENH3 molecules from unbleached centromeres of the same nucleus, indicating a stable incorporation of CENH3 into centromeric nucleosomes. In differentiated endopolyploid nuclei however, the amount of CENH3 at centromeres declines with age.

Citing Articles

Heat stress impairs centromere structure and segregation of meiotic chromosomes in .

Crhak Khaitova L, Mikulkova P, Pecinkova J, Kalidass M, Heckmann S, Lermontova I Elife. 2024; 12.

PMID: 38629825 PMC: 11023694. DOI: 10.7554/eLife.90253.

Transcriptomic Analysis of Radish ( L.) Spontaneous Tumor.

Tkachenko A, Dodueva I, Tvorogova V, Predeus A, Pravdina O, Kuznetsova K Plants (Basel). 2021; 10(5).

PMID: 34063717 PMC: 8147785. DOI: 10.3390/plants10050919.

KINESIN-12E regulates metaphase spindle flux and helps control spindle size in Arabidopsis.

Herrmann A, Livanos P, Zimmermann S, Berendzen K, Rohr L, Lipka E Plant Cell. 2021; 33(1):27-43.

PMID: 33751090 PMC: 8136872. DOI: 10.1093/plcell/koaa003.

Emerging roles of centromeric RNAs in centromere formation and function.

Liu Q, Liu Y, Shi Q, Su H, Wang C, Birchler J Genes Genomics. 2021; 43(3):217-226.

PMID: 33523401 DOI: 10.1007/s13258-021-01041-y.

Deregulated Phosphorylation of CENH3 at Ser65 Affects the Development of Floral Meristems in .

Demidov D, Heckmann S, Weiss O, Rutten T, Dvorak Tomastikova E, Kuhlmann M Front Plant Sci. 2019; 10:928.

PMID: 31404279 PMC: 6671561. DOI: 10.3389/fpls.2019.00928.

References

Hemmerich P, Weidtkamp-Peters S, Hoischen C, Schmiedeberg L, Erliandri I, Diekmann S . Dynamics of inner kinetochore assembly and maintenance in living cells. J Cell Biol. 2008; 180(6):1101-14. PMC: 2290840. DOI: 10.1083/jcb.200710052. View

Silva M, Jansen L . At the right place at the right time: novel CENP-A binding proteins shed light on centromere assembly. Chromosoma. 2009; 118(5):567-74. DOI: 10.1007/s00412-009-0227-3. View

Amor D, Choo K . Neocentromeres: role in human disease, evolution, and centromere study. Am J Hum Genet. 2002; 71(4):695-714. PMC: 378529. DOI: 10.1086/342730. View

Foltz D, Jansen L, Bailey A, Yates 3rd J, Bassett E, Wood S . Centromere-specific assembly of CENP-a nucleosomes is mediated by HJURP. Cell. 2009; 137(3):472-84. PMC: 2747366. DOI: 10.1016/j.cell.2009.02.039. View

Takayama Y, Sato H, Saitoh S, Ogiyama Y, Masuda F, Takahashi K . Biphasic incorporation of centromeric histone CENP-A in fission yeast. Mol Biol Cell. 2007; 19(2):682-90. PMC: 2230595. DOI: 10.1091/mbc.e07-05-0504. View

Allshire R . Centromeres, checkpoints and chromatid cohesion. Curr Opin Genet Dev. 1997; 7(2):264-73. DOI: 10.1016/s0959-437x(97)80137-2. View

NICKLAS R . How cells get the right chromosomes. Science. 1997; 275(5300):632-7. DOI: 10.1126/science.275.5300.632. View

Sanchez-Pulido L, Pidoux A, Ponting C, Allshire R . Common ancestry of the CENP-A chaperones Scm3 and HJURP. Cell. 2009; 137(7):1173-4. PMC: 4397584. DOI: 10.1016/j.cell.2009.06.010. View

Warburton P, Cooke C, Bourassa S, Vafa O, Sullivan B, Stetten G . Immunolocalization of CENP-A suggests a distinct nucleosome structure at the inner kinetochore plate of active centromeres. Curr Biol. 1998; 7(11):901-4. DOI: 10.1016/s0960-9822(06)00382-4. View

10.

Heckmann S, Lermontova I, Berckmans B, De Veylder L, Baumlein H, Schubert I . The E2F transcription factor family regulates CENH3 expression in Arabidopsis thaliana. Plant J. 2011; 68(4):646-56. DOI: 10.1111/j.1365-313X.2011.04715.x. View

11.

Dalal Y, Bui M . Down the rabbit hole of centromere assembly and dynamics. Curr Opin Cell Biol. 2010; 22(3):392-402. PMC: 2989927. DOI: 10.1016/j.ceb.2010.02.005. View

12.

Lermontova I, Fuchs J, Schubert V, Schubert I . Loading time of the centromeric histone H3 variant differs between plants and animals. Chromosoma. 2007; 116(6):507-10. DOI: 10.1007/s00412-007-0122-8. View

13.

You R, Doerner P . Technical advance: spatio-temporal analysis of mitotic activity with a labile cyclin-GUS fusion protein. Plant J. 1999; 20(4):503-8. DOI: 10.1046/j.1365-313x.1999.00620.x. View

14.

Farr C . Centromeres, kinetochores and the segregation of chromosomes. Foreword. Chromosome Res. 2004; 12(6):517-20. DOI: 10.1023/B:CHRO.0000036609.99052.35. View

15.

Amor D, Kalitsis P, Sumer H, Choo K . Building the centromere: from foundation proteins to 3D organization. Trends Cell Biol. 2004; 14(7):359-68. DOI: 10.1016/j.tcb.2004.05.009. View

16.

Schuh M, Lehner C, Heidmann S . Incorporation of Drosophila CID/CENP-A and CENP-C into centromeres during early embryonic anaphase. Curr Biol. 2007; 17(3):237-43. DOI: 10.1016/j.cub.2006.11.051. View

17.

Lee S, Itkin-Ansari P, Levine F . CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells. Aging (Albany NY). 2010; 2(11):785-90. PMC: 3006021. DOI: 10.18632/aging.100220. View

18.

SHELBY R, Monier K, Sullivan K . Chromatin assembly at kinetochores is uncoupled from DNA replication. J Cell Biol. 2000; 151(5):1113-8. PMC: 2174364. DOI: 10.1083/jcb.151.5.1113. View

19.

Maggert K, Karpen G . The activation of a neocentromere in Drosophila requires proximity to an endogenous centromere. Genetics. 2001; 158(4):1615-28. PMC: 1461751. DOI: 10.1093/genetics/158.4.1615. View

20.

Ahmad K, Henikoff S . Centromeres are specialized replication domains in heterochromatin. J Cell Biol. 2001; 153(1):101-10. PMC: 2185517. DOI: 10.1083/jcb.153.1.101. View