Centromeres and Kinetochores of Brassicaceae

Overview

Journal Chromosome Res

Date 2014 May 8

PMID 24801345

Citations 11

Authors

Inna Lermontova

Michael Sandmann

Dmitri Demidov

Affiliations

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Abstract

The centromere-the primary constriction of monocentric chromosomes-is essential for correct segregation of chromosomes during mitosis and meiosis. Centromeric DNA varies between different organisms in sequence composition and extension. The main components of centromeric and pericentromeric DNA of Brassicaceae species are centromeric satellite repeats. Centromeric DNA initiates assembly of the kinetochore, the large protein complex where the spindle fibers attach during nuclear division to pull sister chromatids apart. Kinetochore assembly is initiated by incorporation of the centromeric histone H3 cenH3 into centromeric nucleosomes. The spindle assembly checkpoint acts during mitosis and meiosis at centromeres and maintains genome stability by preventing chromosome segregation before all kinetochores are correctly attached to microtubules. The function of the spindle assembly checkpoint in plants is still poorly understood. Here, we review recent advances of studies on structure and functional importance of centromeric DNA of Brassicaceae, assembly and function of cenH3 in Arabidopsis thaliana and characterization of core SAC proteins of A. thaliana in comparison with non-plant homologues.

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References

Kapusi E, Ma L, Teo C, Hensel G, Himmelbach A, Schubert I . Telomere-mediated truncation of barley chromosomes. Chromosoma. 2011; 121(2):181-90. DOI: 10.1007/s00412-011-0351-8. View

Franzke A, Lysak M, Al-Shehbaz I, Koch M, Mummenhoff K . Cabbage family affairs: the evolutionary history of Brassicaceae. Trends Plant Sci. 2010; 16(2):108-16. DOI: 10.1016/j.tplants.2010.11.005. View

De Rop V, Padeganeh A, Maddox P . CENP-A: the key player behind centromere identity, propagation, and kinetochore assembly. Chromosoma. 2012; 121(6):527-38. PMC: 3501172. DOI: 10.1007/s00412-012-0386-5. View

Barnhart M, Kuich P, Stellfox M, Ward J, Bassett E, Black B . HJURP is a CENP-A chromatin assembly factor sufficient to form a functional de novo kinetochore. J Cell Biol. 2011; 194(2):229-43. PMC: 3144403. DOI: 10.1083/jcb.201012017. View

Dong Q, Han F . Phosphorylation of histone H2A is associated with centromere function and maintenance in meiosis. Plant J. 2012; 71(5):800-9. DOI: 10.1111/j.1365-313X.2012.05029.x. View

Berr A, Pecinka A, Meister A, Kreth G, Fuchs J, Blattner F . Chromosome arrangement and nuclear architecture but not centromeric sequences are conserved between Arabidopsis thaliana and Arabidopsis lyrata. Plant J. 2006; 48(5):771-83. DOI: 10.1111/j.1365-313X.2006.02912.x. View

Burgos-Rivera B, Dawe R . An Arabidopsis tissue-specific RNAi method for studying genes essential to mitosis. PLoS One. 2012; 7(12):e51388. PMC: 3517552. DOI: 10.1371/journal.pone.0051388. View

Ding D, Muthuswamy S, Meier I . Functional interaction between the Arabidopsis orthologs of spindle assembly checkpoint proteins MAD1 and MAD2 and the nucleoporin NUA. Plant Mol Biol. 2012; 79(3):203-16. DOI: 10.1007/s11103-012-9903-4. View

Izuta H, Ikeno M, Suzuki N, Tomonaga T, Nozaki N, Obuse C . Comprehensive analysis of the ICEN (Interphase Centromere Complex) components enriched in the CENP-A chromatin of human cells. Genes Cells. 2006; 11(6):673-84. DOI: 10.1111/j.1365-2443.2006.00969.x. View

10.

Wang M, Tang D, Luo Q, Jin Y, Shen Y, Wang K . BRK1, a Bub1-related kinase, is essential for generating proper tension between homologous kinetochores at metaphase I of rice meiosis. Plant Cell. 2012; 24(12):4961-73. PMC: 3556969. DOI: 10.1105/tpc.112.105874. View

11.

Lee S, Itkin-Ansari P, Levine F . CENP-A, a protein required for chromosome segregation in mitosis, declines with age in islet but not exocrine cells. Aging (Albany NY). 2010; 2(11):785-90. PMC: 3006021. DOI: 10.18632/aging.100220. View

12.

Hayashi T, Fujita Y, Iwasaki O, Adachi Y, Takahashi K, Yanagida M . Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell. 2004; 118(6):715-29. DOI: 10.1016/j.cell.2004.09.002. View

13.

SHELBY R, Monier K, Sullivan K . Chromatin assembly at kinetochores is uncoupled from DNA replication. J Cell Biol. 2000; 151(5):1113-8. PMC: 2174364. DOI: 10.1083/jcb.151.5.1113. View

14.

. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000; 408(6814):796-815. DOI: 10.1038/35048692. View

15.

de Oliveira E, Correia Romeiro N, Ribeiro E, Santa-Catarina C, Oliveira A, Silveira V . Structural and functional characterization of the protein kinase Mps1 in Arabidopsis thaliana. PLoS One. 2012; 7(9):e45707. PMC: 3458904. DOI: 10.1371/journal.pone.0045707. View

16.

Lermontova I, Koroleva O, Rutten T, Fuchs J, Schubert V, Moraes I . Knockdown of CENH3 in Arabidopsis reduces mitotic divisions and causes sterility by disturbed meiotic chromosome segregation. Plant J. 2011; 68(1):40-50. DOI: 10.1111/j.1365-313X.2011.04664.x. View

17.

Hemmerich P, Weidtkamp-Peters S, Hoischen C, Schmiedeberg L, Erliandri I, Diekmann S . Dynamics of inner kinetochore assembly and maintenance in living cells. J Cell Biol. 2008; 180(6):1101-14. PMC: 2290840. DOI: 10.1083/jcb.200710052. View

18.

Thompson H, Schmidt R, Brandes A, Heslop-Harrison J, Dean C . A novel repetitive sequence associated with the centromeric regions of Arabidopsis thaliana chromosomes. Mol Gen Genet. 1996; 253(1-2):247-52. DOI: 10.1007/s004380050319. View

19.

Wieland G, Orthaus S, Ohndorf S, Diekmann S, Hemmerich P . Functional complementation of human centromere protein A (CENP-A) by Cse4p from Saccharomyces cerevisiae. Mol Cell Biol. 2004; 24(15):6620-30. PMC: 444843. DOI: 10.1128/MCB.24.15.6620-6630.2004. View

20.

Obuse C, Yang H, Nozaki N, Goto S, Okazaki T, Yoda K . Proteomics analysis of the centromere complex from HeLa interphase cells: UV-damaged DNA binding protein 1 (DDB-1) is a component of the CEN-complex, while BMI-1 is transiently co-localized with the centromeric region in interphase. Genes Cells. 2004; 9(2):105-20. DOI: 10.1111/j.1365-2443.2004.00705.x. View