Arabidopsis Kinetochore Null2 is an Upstream Component for Centromeric Histone H3 Variant CenH3 Deposition at Centromeres

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2013 Sep 10

PMID 24014547

Citations 47

Authors

Inna Lermontova

Markus Kuhlmann

Swetlana Friedel

Twan Rutten

Stefan Heckmann

Michael Sandmann

Dmitri Demidov

Veit Schubert

Ingo Schubert

Affiliations

Soon will be listed here.

Abstract

The centromeric histone H3 variant cenH3 is an essential centromeric protein required for assembly, maintenance, and proper function of kinetochores during mitosis and meiosis. We identified a kinetochore null2 (KNL2) homolog in Arabidopsis thaliana and uncovered features of its role in cenH3 loading at centromeres. We show that Arabidopsis KNL2 colocalizes with cenH3 and is associated with centromeres during all stages of the mitotic cell cycle, except from metaphase to mid-anaphase. KNL2 is regulated by the proteasome degradation pathway. The KNL2 promoter is mainly active in meristematic tissues, similar to the cenH3 promoter. A knockout mutant for KNL2 shows a reduced level of cenH3 expression and reduced amount of cenH3 protein at chromocenters of meristematic nuclei, anaphase bridges during mitosis, micronuclei in pollen tetrads, and 30% seed abortion. Moreover, knl2 mutant plants display reduced expression of suppressor of variegation 3-9 homologs2, 4, and 9 and reduced DNA methylation, suggesting an impact of KNL2 on the epigenetic environment for centromere maintenance.

Citing Articles

Centromeric localization of αKNL2 and CENP-C proteins in plants depends on their centromere-targeting domain and DNA-binding regions.

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Plant kinetochore complex: composition, function, and regulation.

Xie Y, Wang M, Mo B, Liang C Front Plant Sci. 2024; 15:1467236.

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Exploring Plant Meiosis: Insights from the Kinetochore Perspective.

Zhou K, Zhang C, Niu F, Bai H, Wu D, Deng J Curr Issues Mol Biol. 2023; 45(10):7974-7995.

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