Deregulated Phosphorylation of CENH3 at Ser65 Affects the Development of Floral Meristems in

Overview

Journal Front Plant Sci

Date 2019 Aug 13

PMID 31404279

Citations 5

Authors

Dmitri Demidov

Stefan Heckmann

Oda Weiss

Twan Rutten

Eva Dvorak Tomastikova

Markus Kuhlmann

Patrick Scholl

Celia Maria Municio

Inna Lermontova

Andreas Houben

Affiliations

Soon will be listed here.

Abstract

Several histone variants are posttranslationally phosphorylated. Little is known about phosphorylation of the centromere-specific histone 3 (CENH3) variant in plants. We show that CENH3 of is phosphorylated by Aurora3, predominantly at serine 65. Interaction of Aurora3 and CENH3 was found by immunoprecipitation (IP) in and by bimolecular fluorescence complementation. Western blotting with an anti-CENH3 pS65 antibody showed that CENH3 pS65 is more abundant in flower buds than elsewhere in the plant. Substitution of serine 65 by either alanine or aspartic acid resulted in a range of phenotypic abnormalities, especially in reproductive tissues. We conclude that Aurora3 phosphorylates CENH3 at S65 and that this post-translational modification is required for the proper development of the floral meristem.

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