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Targeted Genome Editing Across Species Using ZFNs and TALENs

Overview
Journal Science
Specialty Science
Date 2011 Jun 25
PMID 21700836
Citations 294
Authors
Andrew J Wood
Te-Wen Lo
Bryan Zeitler
Catherine S Pickle
Edward J Ralston
Andrew H Lee
Rainier Amora
Jeffrey C Miller
Elo Leung
Xiangdong Meng
Lei Zhang
Edward J Rebar
Philip D Gregory
Fyodor D Urnov
Barbara J Meyer
Affiliations
Soon will be listed here.
Abstract

Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

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