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Cloning of Alpha- and Beta-galactosidase Genes from an Extreme Thermophile, Thermus Strain T2, and Their Expression in Thermus Thermophilus HB27

Overview
Journal Appl Environ Microbiol
Specialties Environmental Health
Microbiology
Date 1990 Jul 1
PMID 2167630
Citations 21
Authors
Y Koyama
S Okamoto
K Furukawa
Affiliations
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Abstract

The genes encoding thermostable alpha- and beta-galactosidases from an extremely thermophilic bacterium, Thermus strain T2, were cloned in Escherichia coli. The alpha-galactosidase gene was located just downstream from the beta-galactosidase gene. The genes were introduced into Thermus thermophilus HB27 with the aid of Thermus cryptic plasmid pTT8, and beta-galactosidases were expressed constitutively.

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