Comparative Study of Isoenzyme Formation of Bacterial Beta-galactosidase

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1970 Apr 1

PMID 4908684

Citations 8

Authors

R P Erickson

E Steers Jr

Affiliations

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Abstract

The enzyme beta-galactosidase was studied in crude extracts of Escherichia coli 3300, E. coli grown on a selenium and sulfur medium, Salmonella typhimurium F-lac, Serratia marcescens F-lac, S. marcescens P-lac, Proteus mirabilis F-lac, P. mirabilis P-lac, Aeromonas formicans, and Streptococcus lactis. The isoenzymes could be demonstrated by an alternative histochemical technique. Different isoenzyme patterns were found to be determined by the beta-galactosidase structural gene and not by the cytoplasm within which the beta-galactosidase was formed. In addition, the beta-galactosidases from strains which form isoenzymes were more stable to heat and urea treatments than the enzyme formed by those organisms which produce reduced amounts of, or no, isoenzyme.

Citing Articles

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Molecular basis of isozyme formation of beta-galactosidases in Bacillus stearothermophilus: isolation of two beta-galactosidase genes, bgaA and bgaB.

Hirata H, Negoro S, Okada H J Bacteriol. 1984; 160(1):9-14.

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Involvement of sulfhydryl groups in the -galactosidase of Streptococcus lactis.

McFeters G, Sandine W, Elliker P J Bacteriol. 1971; 108(1):599-600.

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The synthesis of -galactosidase in Escherichia coli in the presence of methionine analogues.

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References

Alpers D, Steers Jr E, Shifrin S, TOMKINS G . Isozymes of the lactose operon of Escherichia coli. Ann N Y Acad Sci. 1968; 151(1):545-55. DOI: 10.1111/j.1749-6632.1968.tb11914.x. View

Colby Jr C, HU A . Purification and comparison of the beta-galactosidase synthesized by Escherichia coli F-lac and Proteus mirabilis F-lac. Biochim Biophys Acta. 1968; 157(1):167-77. DOI: 10.1016/0005-2787(68)90275-x. View

MONOD J, Cohn M . [Biosynthesis induced by enzymes; enzymatic adaptation]. Adv Enzymol Relat Subj Biochem. 1952; 13:67-119. View

Colwell R, Liston J . Taxonomic relationships among the pseudomonads. J Bacteriol. 1961; 82:1-14. PMC: 279106. DOI: 10.1128/jb.82.1.1-14.1961. View

Pearson B, Wolf P, Vazquez J . A COMPARATIVE STUDY OF A SERIES OF NEW INDOLYL COMPOUNDS TO LOCALIZE BETA-GALACTOSIDASE IN TISSUES. Lab Invest. 1963; 12:1249-59. View

Falkow S, Wohlhieter J, CITARELLA R, Baron L . TRANSFER OF EPISOMIC ELEMENTS TO PROTEUS. I. TRANSFER OF F-LINKED CHROMOSOMAL DETERMINANTS. J Bacteriol. 1964; 87:209-19. PMC: 276980. DOI: 10.1128/jb.87.1.209-219.1964. View

Wohlhieter J, Falkow S, CITARELLA R, Baron L . CHARACTERIZATION OF DNA FROM A PROTEUS STRAIN HARBORING AN EPISOME. J Mol Biol. 1964; 9:576-88. DOI: 10.1016/s0022-2836(64)80228-x. View

Appel S, Alpers D, Tomkins G . MULTIPLE MOLECULAR FORMS OF BETA-GALACTOSIDASE. J Mol Biol. 1965; 11:12-22. DOI: 10.1016/s0022-2836(65)80167-x. View

CITTI J, Sandine W, Elliker P . BETA-GALACTOSIDASE OF STREPTOCOCCUS LACTIS. J Bacteriol. 1965; 89:937-42. PMC: 277575. DOI: 10.1128/jb.89.4.937-942.1965. View

10.

CRAVEN G, Steers Jr E, ANFINSEN C . PURIFICATION, COMPOSITION, AND MOLECULAR WEIGHT OF THE BETA-GALACTOSIDASE OF ESCHERICHIA COLI K12. J Biol Chem. 1965; 240:2468-77. View

11.

Rohlfing S, Crawford I . Purification and characterization of the beta-galactosidase of Aeromonas formicans. J Bacteriol. 1966; 91(3):1085-97. PMC: 316000. DOI: 10.1128/jb.91.3.1085-1097.1966. View

12.

McFeters G, Sandine W, Elliker P . Purification and properties of Streptococcus lactis beta-galactosidase. J Bacteriol. 1967; 93(3):914-9. PMC: 276535. DOI: 10.1128/jb.93.3.914-919.1967. View

13.

Huber R, Segel I, Criddle R . Growth of Escherichia coli on selenate. Biochim Biophys Acta. 1967; 141(3):573-86. DOI: 10.1016/0304-4165(67)90186-9. View

14.

Huber R, Criddle R . The isolation and properties of beta-galactosidase from Escherichia coli grown on sodium selenate. Biochim Biophys Acta. 1967; 141(3):587-99. DOI: 10.1016/0304-4165(67)90187-0. View

15.

Colby Jr C, HU A . The regulation of the synthesis of beta-galactosidase in Proteus mirabilis F-lac. Biochim Biophys Acta. 1968; 157(1):149-58. DOI: 10.1016/0005-2787(68)90273-6. View

16.

Colby Jr C, Martin F, HU A . Catabolite repression of the synthesis of beta-galactosidase in Proteus mirabilis F-lac. Biochim Biophys Acta. 1968; 157(1):159-66. DOI: 10.1016/0005-2787(68)90274-8. View

17.

MARCHESI S, Steers Jr E, Shifrin S . Purification and characterization of the multiple forms of beta-galactosidase of Escherichia coli. Biochim Biophys Acta. 1969; 181(1):20-34. DOI: 10.1016/0005-2795(69)90223-2. View