Molecular Cloning of the PyrE Gene from the Extreme Thermophile Thermus Flavus

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1995 Aug 1

PMID 7635839

Citations 4

Authors

V Vonstein

S P Johnson

H Yu

M J Casadaban

N C Pagratis

J M Weber

D C Demirjian

Affiliations

Soon will be listed here.

Abstract

Mutants of the extreme thermophile Thermus flavus in the pyrimidine biosynthetic pathway (Pyr-) were isolated by resistance to 5-fluoroorotic acid. The pyrE gene, which codes for the orotate phosphoribosyltransferase, was cloned by recombination with one of the isolated Pyr- T. flavus mutant strains. It was subcloned by complementation of an Escherichia coli pyrE mutant strain and was sequenced. The deduced polypeptide sequence extends over 183 amino acids. Several independent Pyr- mutations were mapped within the pyrE locus by recombination with fragments of the cloned gene.

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References

Koyama Y, Arikawa Y, Furukawa K . A plasmid vector for an extreme thermophile, Thermus thermophilus. FEMS Microbiol Lett. 1990; 60(1-2):97-101. DOI: 10.1016/0378-1097(90)90352-q. View

Weber J, Johnson S, Vonstein V, Casadaban M, Demirjian D . A chromosome integration system for stable gene transfer into Thermus flavus. Biotechnology (N Y). 1995; 13(3):271-5. DOI: 10.1038/nbt0395-271. View

Dekker K, Yamagata H, Sakaguchi K, Udaka S . Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases. J Bacteriol. 1991; 173(10):3078-83. PMC: 207900. DOI: 10.1128/jb.173.10.3078-3083.1991. View

Nishiyama M, Horinouchi S, Beppu T . Characterization of an operon encoding succinyl-CoA synthetase and malate dehydrogenase from Thermus flavus AT-62 and its expression in Escherichia coli. Mol Gen Genet. 1991; 226(1-2):1-9. DOI: 10.1007/BF00273580. View

Yeh M, Trela J . Purification and characterization of a repressible alkaline phosphatase from Thermus aquaticus. J Biol Chem. 1976; 251(10):3134-9. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Casadaban M, Martinez-Arias A, Shapira S, Chou J . Beta-galactosidase gene fusions for analyzing gene expression in escherichia coli and yeast. Methods Enzymol. 1983; 100:293-308. DOI: 10.1016/0076-6879(83)00063-4. View

Matsumura M, Aiba S . Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus. J Biol Chem. 1985; 260(28):15298-303. View

Liao H, McKenzie T, Hageman R . Isolation of a thermostable enzyme variant by cloning and selection in a thermophile. Proc Natl Acad Sci U S A. 1986; 83(3):576-80. PMC: 322906. DOI: 10.1073/pnas.83.3.576. View

10.

Koyama Y, Hoshino T, Tomizuka N, Furukawa K . Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp. J Bacteriol. 1986; 166(1):338-40. PMC: 214599. DOI: 10.1128/jb.166.1.338-340.1986. View

11.

Hershey H, Taylor M . Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes. Gene. 1986; 43(3):287-93. DOI: 10.1016/0378-1119(86)90218-0. View

12.

Gibson T, Rosenthal A, Waterston R . Lorist6, a cosmid vector with BamHI, NotI, ScaI and HindIII cloning sites and altered neomycin phosphotransferase gene expression. Gene. 1987; 53(2-3):283-6. DOI: 10.1016/0378-1119(87)90017-5. View

13.

Koyama Y, Furukawa K . Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells. J Bacteriol. 1990; 172(6):3490-5. PMC: 209164. DOI: 10.1128/jb.172.6.3490-3495.1990. View

14.

Koyama Y, Okamoto S, Furukawa K . Cloning of alpha- and beta-galactosidase genes from an extreme thermophile, Thermus strain T2, and their expression in Thermus thermophilus HB27. Appl Environ Microbiol. 1990; 56(7):2251-4. PMC: 184593. DOI: 10.1128/aem.56.7.2251-2254.1990. View

15.

Nicholls D, Sundaram T, Atkinson T, Minton N . Cloning and nucleotide sequences of the mdh and sucD genes from Thermus aquaticus B. FEMS Microbiol Lett. 1990; 58(1):7-14. DOI: 10.1016/0378-1097(90)90093-6. View

16.

Lauer G, Rudd E, McKay D, Ally A, Ally D, Backman K . Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog of Escherichia coli DNA ligase. J Bacteriol. 1991; 173(16):5047-53. PMC: 208194. DOI: 10.1128/jb.173.16.5047-5053.1991. View

17.

Berges T, Barreau C . Isolation of uridine auxotrophs from Trichoderma reesei and efficient transformation with the cloned ura3 and ura5 genes. Curr Genet. 1991; 19(5):359-65. DOI: 10.1007/BF00309596. View

18.

Silar P, Thiele D . New shuttle vectors for direct cloning in Saccharomyces cerevisiae. Gene. 1991; 104(1):99-102. DOI: 10.1016/0378-1119(91)90472-n. View

19.

Mather M, Fee J . Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene. Appl Environ Microbiol. 1992; 58(1):421-5. PMC: 195228. DOI: 10.1128/aem.58.1.421-425.1992. View

20.

Kwon-Chung K, Varma A, Edman J, Bennett J . Selection of ura5 and ura3 mutants from the two varieties of Cryptococcus neoformans on 5-fluoroorotic acid medium. J Med Vet Mycol. 1992; 30(1):61-9. View