The Truncated Phage Lysin CHAP(k) Eliminates Staphylococcus Aureus in the Nares of Mice

Overview

Journal Bioeng Bugs

Specialties Biomedical Engineering
Biotechnology
Microbiology

Date 2011 Apr 7

PMID 21468207

Citations 47

Authors

Mark Fenton

Pat G Casey

Colin Hill

Cormac Gm Gahan

R Paul Ross

Olivia McAuliffe

Jim OMahony

Fiona Maher

Aidan Coffey

Affiliations

Soon will be listed here.

Abstract

The endolysin LysK derived from staphylococcal phage K has previously been shown to have two enzymatic domains, one of which is an N-acetylmuramoyl-L-alanine amidase and the other a cysteine/histidine-dependant amidohydrolase/peptidase designated CHAP(k). The latter, when cloned as a single-domain truncated enzyme, is conveniently overexpressed in a highly-soluble form. This enzyme was shown to be highly active in vitro against live cell suspensions of S. aureus. In the current study, the IVIS imaging system was used to demonstrate the effective elimination of a lux labeled S. aureus from the nares of BALB/c mice.

Citing Articles

Phage-Derived Endolysins Against Resistant Staphylococcus spp.: A Review of Features, Antibacterial Activities, and Recent Applications.

Golban M, Charostad J, Kazemian H, Heidari H Infect Dis Ther. 2024; 14(1):13-57.

PMID: 39549153 PMC: 11782739. DOI: 10.1007/s40121-024-01069-z.

The Synergistic and Chimeric Mechanism of Bacteriophage Endolysins: Opportunities for Application in Biotherapeutics, Food, and Health Sectors.

Behera M, De S, Ghorai S Probiotics Antimicrob Proteins. 2024; .

PMID: 39508962 DOI: 10.1007/s12602-024-10394-1.

Decoding the Structure-Function Relationship of the Muramidase Domain in E. coli O157.H7 Bacteriophage Endolysin: A Potential Building Block for Chimeric Enzybiotics.

Javid M, Shahverdi A, Ghasemi A, Moosavi-Movahedi A, Ebrahim-Habibi A, Sepehrizadeh Z Protein J. 2024; 43(3):522-543.

PMID: 38662183 DOI: 10.1007/s10930-024-10195-z.

What's in a Name? An Overview of the Proliferating Nomenclature in the Field of Phage Lysins.

Vazquez R, Briers Y Cells. 2023; 12(15).

PMID: 37566095 PMC: 10417350. DOI: 10.3390/cells12152016.

Therapeutic potential of bacteriophage endolysins for infections caused by Gram-positive bacteria.

Liu H, Hu Z, Li M, Yang Y, Lu S, Rao X J Biomed Sci. 2023; 30(1):29.

PMID: 37101261 PMC: 10131408. DOI: 10.1186/s12929-023-00919-1.

References

Grandgirard D, Loeffler J, Fischetti V, Leib S . Phage lytic enzyme Cpl-1 for antibacterial therapy in experimental pneumococcal meningitis. J Infect Dis. 2008; 197(11):1519-22. DOI: 10.1086/587942. View

Jado I, Lopez R, Garcia E, Fenoll A, Casal J, Garcia P . Phage lytic enzymes as therapy for antibiotic-resistant Streptococcus pneumoniae infection in a murine sepsis model. J Antimicrob Chemother. 2003; 52(6):967-73. DOI: 10.1093/jac/dkg485. View

Nelson D, Loomis L, Fischetti V . Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme. Proc Natl Acad Sci U S A. 2001; 98(7):4107-12. PMC: 31187. DOI: 10.1073/pnas.061038398. View

Fenton M, Ross P, McAuliffe O, OMahony J, Coffey A . Recombinant bacteriophage lysins as antibacterials. Bioeng Bugs. 2011; 1(1):9-16. PMC: 3035150. DOI: 10.4161/bbug.1.1.9818. View

Manoharadas S, Witte A, Blasi U . Antimicrobial activity of a chimeric enzybiotic towards Staphylococcus aureus. J Biotechnol. 2008; 139(1):118-23. DOI: 10.1016/j.jbiotec.2008.09.003. View

Becker S, Foster-Frey J, Donovan D . The phage K lytic enzyme LysK and lysostaphin act synergistically to kill MRSA. FEMS Microbiol Lett. 2008; 287(2):185-91. DOI: 10.1111/j.1574-6968.2008.01308.x. View

Daniel A, Euler C, Collin M, Chahales P, Gorelick K, Fischetti V . Synergism between a novel chimeric lysin and oxacillin protects against infection by methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2010; 54(4):1603-12. PMC: 2849374. DOI: 10.1128/AAC.01625-09. View

Loessner M, Maier S, Wendlinger G, Scherer S . Three Bacillus cereus bacteriophage endolysins are unrelated but reveal high homology to cell wall hydrolases from different bacilli. J Bacteriol. 1997; 179(9):2845-51. PMC: 179044. DOI: 10.1128/jb.179.9.2845-2851.1997. View

Zimmer M, Vukov N, Scherer S, Loessner M . The murein hydrolase of the bacteriophage phi3626 dual lysis system is active against all tested Clostridium perfringens strains. Appl Environ Microbiol. 2002; 68(11):5311-7. PMC: 129905. DOI: 10.1128/AEM.68.11.5311-5317.2002. View

10.

Entenza J, Loeffler J, Grandgirard D, Fischetti V, Moreillon P . Therapeutic effects of bacteriophage Cpl-1 lysin against Streptococcus pneumoniae endocarditis in rats. Antimicrob Agents Chemother. 2005; 49(11):4789-92. PMC: 1280127. DOI: 10.1128/AAC.49.11.4789-4792.2005. View

11.

Yu J, Wu J, Francis K, Purchio T, Kadurugamuwa J . Monitoring in vivo fitness of rifampicin-resistant Staphylococcus aureus mutants in a mouse biofilm infection model. J Antimicrob Chemother. 2005; 55(4):528-34. DOI: 10.1093/jac/dki053. View

12.

Loessner M . Bacteriophage endolysins--current state of research and applications. Curr Opin Microbiol. 2005; 8(4):480-7. DOI: 10.1016/j.mib.2005.06.002. View

13.

Cheng Q, Nelson D, Zhu S, Fischetti V . Removal of group B streptococci colonizing the vagina and oropharynx of mice with a bacteriophage lytic enzyme. Antimicrob Agents Chemother. 2004; 49(1):111-7. PMC: 538902. DOI: 10.1128/AAC.49.1.111-117.2005. View

14.

OFlaherty S, Coffey A, Meaney W, Fitzgerald G, Ross R . The recombinant phage lysin LysK has a broad spectrum of lytic activity against clinically relevant staphylococci, including methicillin-resistant Staphylococcus aureus. J Bacteriol. 2005; 187(20):7161-4. PMC: 1251611. DOI: 10.1128/JB.187.20.7161-7164.2005. View

15.

Rashel M, Uchiyama J, Ujihara T, Uehara Y, Kuramoto S, Sugihara S . Efficient elimination of multidrug-resistant Staphylococcus aureus by cloned lysin derived from bacteriophage phi MR11. J Infect Dis. 2007; 196(8):1237-47. DOI: 10.1086/521305. View

16.

Fischetti V . Bacteriophage endolysins: a novel anti-infective to control Gram-positive pathogens. Int J Med Microbiol. 2010; 300(6):357-62. PMC: 3666336. DOI: 10.1016/j.ijmm.2010.04.002. View

17.

Kadurugamuwa J, Sin L, Yu J, Francis K, Purchio T, Contag P . Noninvasive optical imaging method to evaluate postantibiotic effects on biofilm infection in vivo. Antimicrob Agents Chemother. 2004; 48(6):2283-7. PMC: 415634. DOI: 10.1128/AAC.48.6.2283-2287.2004. View

18.

Sass P, Bierbaum G . Lytic activity of recombinant bacteriophage phi11 and phi12 endolysins on whole cells and biofilms of Staphylococcus aureus. Appl Environ Microbiol. 2006; 73(1):347-52. PMC: 1797112. DOI: 10.1128/AEM.01616-06. View

19.

Garcia P, Lopez R, Ronda C, Garcia E, Tomasz A . Mechanism of phage-induced lysis in pneumococci. J Gen Microbiol. 1983; 129(2):479-87. DOI: 10.1099/00221287-129-2-479. View

20.

McCullers J, Karlstrom A, Iverson A, Loeffler J, Fischetti V . Novel strategy to prevent otitis media caused by colonizing Streptococcus pneumoniae. PLoS Pathog. 2007; 3(3):e28. PMC: 1829406. DOI: 10.1371/journal.ppat.0030028. View