Alpha 2.0
Login Register
» Articles » PMID: 15155235

Noninvasive Optical Imaging Method to Evaluate Postantibiotic Effects on Biofilm Infection in Vivo

Overview
Journal Antimicrob Agents Chemother
Publisher American Society for Microbiology
Specialty Pharmacology
Date 2004 May 25
PMID 15155235
Citations 24
Authors
Jagath L Kadurugamuwa
Lin V Sin
Jun Yu
Kevin P Francis
Tony F Purchio
Pamela R Contag
Affiliations
Soon will be listed here.
Abstract

Eradication of Staphylococcus aureus biofilms after rifampin treatment was tested in a mouse model of device-related infection by using biophotonic imaging. Following treatment, the bioluminescent signals decreased to undetectable levels, irrespective of the age of the biofilm. After the final treatment, the signals rebounded in a time-dependent manner and reached those for the untreated mice. Readministration of rifampin was unsuccessful in eradicating reestablished infections, with the rifampin MICs for such bacteria being increased and with the bacteria having point mutations in the rpoB gene.

Citing Articles

Utilization of In Vivo Imaging System to Study Staphylococcal Sepsis and Septic Arthritis Progression in Mouse Model.

Deshmukh M, Hu Z, Mohammad M, Jin T Pathogens. 2024; 13(8).

PMID: 39204252 PMC: 11357683. DOI: 10.3390/pathogens13080652.

Extended local release and improved bacterial eradication by adding rifampicin to a biphasic ceramic carrier containing gentamicin or vancomycin.

Sebastian S, Tandberg F, Liu Y, Raina D, Tagil M, Collin M Bone Joint Res. 2022; 11(11):787-802.

PMID: 36349950 PMC: 9680205. DOI: 10.1302/2046-3758.1111.BJR-2022-0101.R1.

Role of Phenol-Soluble Modulins in Staphylococcus epidermidis Biofilm Formation and Infection of Indwelling Medical Devices.

Le K, Villaruz A, Zheng Y, He L, Fisher E, Nguyen T J Mol Biol. 2019; 431(16):3015-3027.

PMID: 30954574 PMC: 10999989. DOI: 10.1016/j.jmb.2019.03.030.

Emergency 1-stage anterior approach for cervical spine infection complicated by epidural abscess.

Li H, Chen Z, Yong Z, Li X, Huang Y, Wu D Medicine (Baltimore). 2017; 96(26):e7301.

PMID: 28658132 PMC: 5500054. DOI: 10.1097/MD.0000000000007301.

Combinatory antibiotic therapy increases rate of bacterial kill but not final outcome in a novel mouse model of Staphylococcus aureus spinal implant infection.

Hu Y, Hegde V, Johansen D, Loftin A, Dworsky E, Zoller S PLoS One. 2017; 12(2):e0173019.

PMID: 28245229 PMC: 5330510. DOI: 10.1371/journal.pone.0173019.

References
1.
Lee S, Camilli A . Novel approaches to monitor bacterial gene expression in infected tissue and host. Curr Opin Microbiol. 2000; 3(1):97-101. DOI: 10.1016/s1369-5274(99)00058-2. View
2.
Siragusa G, Nawotka K, Spilman S, Contag P, Contag C . Real-time monitoring of Escherichia coli O157:H7 adherence to beef carcass surface tissues with a bioluminescent reporter. Appl Environ Microbiol. 1999; 65(4):1738-45. PMC: 91245. DOI: 10.1128/AEM.65.4.1738-1745.1999. View
3.
Rocchetta H, Boylan C, FOLEY J, Iversen P, LeTourneau D, McMillian C . Validation of a noninvasive, real-time imaging technology using bioluminescent Escherichia coli in the neutropenic mouse thigh model of infection. Antimicrob Agents Chemother. 2000; 45(1):129-37. PMC: 90250. DOI: 10.1128/AAC.45.1.129-137.2001. View
4.
Francis K, Yu J, Joh D, Hawkinson M, Xiao G, Purchio T . Visualizing pneumococcal infections in the lungs of live mice using bioluminescent Streptococcus pneumoniae transformed with a novel gram-positive lux transposon. Infect Immun. 2001; 69(5):3350-8. PMC: 98294. DOI: 10.1128/IAI.69.5.3350-3358.2001. View
5.
Hamblin M, ODonnell D, Murthy N, Contag C, Hasan T . Rapid control of wound infections by targeted photodynamic therapy monitored by in vivo bioluminescence imaging. Photochem Photobiol. 2002; 75(1):51-7. DOI: 10.1562/0031-8655(2002)075<0051:rcowib>2.0.co;2. View