Purified Mesenchymal Stem Cells Are an Efficient Source for IPS Cell Induction

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Mar 18

PMID 21412425

Citations 30

Authors

Kunimichi Niibe

Yoshimi Kawamura

Daisuke Araki

Satoru Morikawa

Kyoko Miura

Sadafumi Suzuki

Shigeto Shimmura

Takehiko Sunabori

Yo Mabuchi

Yasuo Nagai

Taneaki Nakagawa

Hideyuki Okano

Yumi Matsuzaki

Affiliations

Soon will be listed here.

Abstract

Background: Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by the forced expression of defined transcription factors. Although most somatic cells are capable of acquiring pluripotency with minimal gene transduction, the poor efficiency of cell reprogramming and the uneven quality of iPS cells are still important problems. In particular, the choice of cell type most suitable for inducing high-quality iPS cells remains unclear.

Methodology/principal Findings: Here, we generated iPS cells from PDGFRα+ Sca-1+ (PαS) adult mouse mesenchymal stem cells (MSCs) and PDGFRα⁻ Sca-1⁻ osteo-progenitors (OP cells), and compared the induction efficiency and quality of individual iPS clones. MSCs had a higher reprogramming efficiency compared with OP cells and Tail Tip Fibroblasts (TTFs). The iPS cells induced from MSCs by Oct3/4, Sox2, and Klf4 appeared to be the closest equivalent to ES cells by DNA microarray gene profile and germline-transmission efficiency.

Conclusions/significance: Our findings suggest that a purified source of undifferentiated cells from adult tissue can produce high-quality iPS cells. In this context, prospectively enriched MSCs are a promising candidate for the efficient generation of high-quality iPS cells.

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References

Okita K, Ichisaka T, Yamanaka S . Generation of germline-competent induced pluripotent stem cells. Nature. 2007; 448(7151):313-7. DOI: 10.1038/nature05934. View

Morikawa S, Mabuchi Y, Kubota Y, Nagai Y, Niibe K, Hiratsu E . Prospective identification, isolation, and systemic transplantation of multipotent mesenchymal stem cells in murine bone marrow. J Exp Med. 2009; 206(11):2483-96. PMC: 2768869. DOI: 10.1084/jem.20091046. View

Kim J, Sebastiano V, Wu G, Arauzo-Bravo M, Sasse P, Gentile L . Oct4-induced pluripotency in adult neural stem cells. Cell. 2009; 136(3):411-9. DOI: 10.1016/j.cell.2009.01.023. View

Takahashi K, Yamanaka S . Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-76. DOI: 10.1016/j.cell.2006.07.024. View

Stadtfeld M, Apostolou E, Akutsu H, Fukuda A, Follett P, Natesan S . Aberrant silencing of imprinted genes on chromosome 12qF1 in mouse induced pluripotent stem cells. Nature. 2010; 465(7295):175-81. PMC: 3987905. DOI: 10.1038/nature09017. View

Nakagawa M, Koyanagi M, Tanabe K, Takahashi K, Ichisaka T, Aoi T . Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts. Nat Biotechnol. 2007; 26(1):101-6. DOI: 10.1038/nbt1374. View

FRIEDENSTEIN A, Deriglasova U, Kulagina N, Panasuk A, Rudakowa S, Luria E . Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method. Exp Hematol. 1974; 2(2):83-92. View

Takahashi K, Okita K, Nakagawa M, Yamanaka S . Induction of pluripotent stem cells from fibroblast cultures. Nat Protoc. 2007; 2(12):3081-9. DOI: 10.1038/nprot.2007.418. View

Aoi T, Yae K, Nakagawa M, Ichisaka T, Okita K, Takahashi K . Generation of pluripotent stem cells from adult mouse liver and stomach cells. Science. 2008; 321(5889):699-702. DOI: 10.1126/science.1154884. View

10.

Pittenger M, Mackay A, Beck S, Jaiswal R, Douglas R, Mosca J . Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284(5411):143-7. DOI: 10.1126/science.284.5411.143. View

11.

Mitsui K, Tokuzawa Y, Itoh H, Segawa K, Murakami M, Takahashi K . The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell. 2003; 113(5):631-42. DOI: 10.1016/s0092-8674(03)00393-3. View

12.

TARKOWSKI A . Mouse chimaeras revisited: recollections and reflections. Int J Dev Biol. 1998; 42(7):903-8. View

13.

Fusaki N, Ban H, Nishiyama A, Saeki K, Hasegawa M . Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome. Proc Jpn Acad Ser B Phys Biol Sci. 2009; 85(8):348-62. PMC: 3621571. DOI: 10.2183/pjab.85.348. View

14.

Miura K, Okada Y, Aoi T, Okada A, Takahashi K, Okita K . Variation in the safety of induced pluripotent stem cell lines. Nat Biotechnol. 2009; 27(8):743-5. DOI: 10.1038/nbt.1554. View

15.

Morikawa S, Mabuchi Y, Niibe K, Suzuki S, Nagoshi N, Sunabori T . Development of mesenchymal stem cells partially originate from the neural crest. Biochem Biophys Res Commun. 2009; 379(4):1114-9. DOI: 10.1016/j.bbrc.2009.01.031. View

16.

Prockop D . Marrow stromal cells as stem cells for nonhematopoietic tissues. Science. 1997; 276(5309):71-4. DOI: 10.1126/science.276.5309.71. View

17.

Stadtfeld M, Brennand K, Hochedlinger K . Reprogramming of pancreatic beta cells into induced pluripotent stem cells. Curr Biol. 2008; 18(12):890-4. PMC: 2819222. DOI: 10.1016/j.cub.2008.05.010. View

18.

Wood S, Allen N, Rossant J, Auerbach A, Nagy A . Non-injection methods for the production of embryonic stem cell-embryo chimaeras. Nature. 1993; 365(6441):87-9. DOI: 10.1038/365087a0. View

19.

Stadtfeld M, Nagaya M, Utikal J, Weir G, Hochedlinger K . Induced pluripotent stem cells generated without viral integration. Science. 2008; 322(5903):945-9. PMC: 3987909. DOI: 10.1126/science.1162494. View

20.

Kim J, Zaehres H, Wu G, Gentile L, Ko K, Sebastiano V . Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors. Nature. 2008; 454(7204):646-50. DOI: 10.1038/nature07061. View