Heterogeneous Immunoassays in Microfluidic Format Using Fluorescence Detection with Integrated Amorphous Silicon Photodiodes

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2011 Mar 16

PMID 21403847

Citations 4

Authors

A T Pereira

P Novo

D M F Prazeres

V Chu

J P Conde

Affiliations

Soon will be listed here.

Abstract

Miniaturization of immunoassays through microfluidic technology has the potential to decrease the time and the quantity of reactants required for analysis, together with the potential of achieving multiplexing and portability. A lab-on-chip system incorporating a thin-film amorphous silicon (a-Si:H) photodiode microfabricated on a glass substrate with a thin-film amorphous silicon-carbon alloy directly deposited above the photodiode and acting as a fluorescence filter is integrated with a polydimethylsiloxane-based microfluidic network for the direct detection of antibody-antigen molecular recognition reactions using fluorescence. The model immunoassay used consists of primary antibody adsorption to the microchannel walls followed by its recognition by a secondary antibody labeled with a fluorescent quantum-dot tag. The conditions for the flow-through analysis in the microfluidic format were defined and the total assay time was 30 min. Specific molecular recognition was quantitatively detected. The measurements made with the a-Si:H photodiode are consistent with that obtained with a fluorescence microscope and both show a linear dependence on the antibody concentration in the nanomolar-micromolar range.

Citing Articles

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals.

Hayat Khan A, Noordin R Eur J Clin Microbiol Infect Dis. 2019; 39(1):19-30.

PMID: 31428897 PMC: 7087738. DOI: 10.1007/s10096-019-03680-2.

Thin Film Differential Photosensor for Reduction of Temperature Effects in Lab-on-Chip Applications.

de Cesare G, Carpentiero M, Nascetti A, Caputo D Sensors (Basel). 2016; 16(2):267.

PMID: 26907292 PMC: 4801643. DOI: 10.3390/s16020267.

A lateral electrophoretic flow diagnostic assay.

Lin R, Skandarajah A, Gerver R, Neira H, Fletcher D, Herr A Lab Chip. 2015; 15(6):1488-96.

PMID: 25608872 PMC: 4383188. DOI: 10.1039/c4lc01370k.

An integrated microfluidic device for rapid serodiagnosis of amebiasis.

Zhao W, Zhang L, Jing W, Liu S, Tachibana H, Cheng X Biomicrofluidics. 2014; 7(1):11101.

PMID: 24403984 PMC: 3592878. DOI: 10.1063/1.4793222.

References

Choi J, Oh K, Thomas J, Heineman W, Halsall H, Nevin J . An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities. Lab Chip. 2004; 2(1):27-30. DOI: 10.1039/b107540n. View

Panini N, Messina G, Salinas E, Fernandez H, Raba J . Integrated microfluidic systems with an immunosensor modified with carbon nanotubes for detection of prostate specific antigen (PSA) in human serum samples. Biosens Bioelectron. 2007; 23(7):1145-51. DOI: 10.1016/j.bios.2007.11.003. View

Parsa H, Chin C, Mongkolwisetwara P, Lee B, Wang J, Sia S . Effect of volume- and time-based constraints on capture of analytes in microfluidic heterogeneous immunoassays. Lab Chip. 2008; 8(12):2062-70. DOI: 10.1039/b813350f. View

Dodge A, Fluri K, Verpoorte E, de Rooij N . Electrokinetically driven microfluidic chips with surface-modified chambers for heterogeneous immunoassays. Anal Chem. 2001; 73(14):3400-9. DOI: 10.1021/ac0015366. View

Heyries K, Loughran M, Hoffmann D, Homsy A, Blum L, Marquette C . Microfluidic biochip for chemiluminescent detection of allergen-specific antibodies. Biosens Bioelectron. 2008; 23(12):1812-8. DOI: 10.1016/j.bios.2008.02.025. View

Messina G, Panini N, Martinez N, Raba J . Microfluidic immunosensor design for the quantification of interleukin-6 in human serum samples. Anal Biochem. 2008; 380(2):262-7. DOI: 10.1016/j.ab.2008.05.055. View

Chiem N, Harrison D . Microchip-based capillary electrophoresis for immunoassays: analysis of monoclonal antibodies and theophylline. Anal Chem. 1997; 69(3):373-8. DOI: 10.1021/ac9606620. View

Self C, Cook D . Advances in immunoassay technology. Curr Opin Biotechnol. 1996; 7(1):60-5. DOI: 10.1016/s0958-1669(96)80096-6. View

Srinivasan V, Pamula V, Fair R . An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids. Lab Chip. 2004; 4(4):310-5. DOI: 10.1039/b403341h. View

10.

Ihara M, Yoshikawa A, Wu Y, Takahashi H, Mawatari K, Shimura K . Micro OS-ELISA: Rapid noncompetitive detection of a small biomarker peptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device. Lab Chip. 2009; 10(1):92-100. DOI: 10.1039/b915516c. View

11.

Sung W, Chang C, Makamba H, Chen S . Long-term affinity modification on poly(dimethylsiloxane) substrate and its application for ELISA analysis. Anal Chem. 2008; 80(5):1529-35. DOI: 10.1021/ac7020618. View

12.

Huang Y, Zhao S, Shi M, Liu Y . Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis. Anal Biochem. 2009; 399(1):72-7. PMC: 2831779. DOI: 10.1016/j.ab.2009.11.036. View

13.

Shin K, Lee S, Han K, Kim S, Yang E, Park J . Amplification of fluorescence with packed beads to enhance the sensitivity of miniaturized detection in microfluidic chip. Biosens Bioelectron. 2006; 22(9-10):2261-7. DOI: 10.1016/j.bios.2006.11.011. View

14.

Dandin M, Abshire P, Smela E . Optical filtering technologies for integrated fluorescence sensors. Lab Chip. 2007; 7(8):955-77. DOI: 10.1039/b704008c. View

15.

Kamei T, Toriello N, Lagally E, Blazej R, Scherer J, Street R . Microfluidic genetic analysis with an integrated a-Si:H detector. Biomed Microdevices. 2005; 7(2):147-52. DOI: 10.1007/s10544-005-1595-y. View

16.

Kuswandi B, Nuriman , Huskens J, Verboom W . Optical sensing systems for microfluidic devices: a review. Anal Chim Acta. 2007; 601(2):141-55. DOI: 10.1016/j.aca.2007.08.046. View

17.

Sun S, Yang M, Kostov Y, Rasooly A . ELISA-LOC: lab-on-a-chip for enzyme-linked immunodetection. Lab Chip. 2010; 10(16):2093-100. DOI: 10.1039/c003994b. View

18.

Nashida N, Satoh W, Fukuda J, Suzuki H . Electrochemical immunoassay on a microfluidic device with sequential injection and flushing functions. Biosens Bioelectron. 2007; 22(12):3167-73. DOI: 10.1016/j.bios.2007.02.010. View

19.

Laiwattanapaisal W, Songjaroen T, Maturos T, Lomas T, Sappat A, Tuantranont A . On-chip immunoassay for determination of urinary albumin. Sensors (Basel). 2012; 9(12):10066-79. PMC: 3267210. DOI: 10.3390/s91210066. View

20.

Hu S, Ren X, Bachman M, Sims C, Li G, Allbritton N . Tailoring the surface properties of poly(dimethylsiloxane) microfluidic devices. Langmuir. 2005; 20(13):5569-74. DOI: 10.1021/la049974l. View