Large Intergenic Non-coding RNA-RoR Modulates Reprogramming of Human Induced Pluripotent Stem Cells

Overview

Journal Nat Genet

Specialty Genetics

Date 2010 Nov 9

PMID 21057500

Citations 529

Authors

Sabine Loewer

Moran N Cabili

Mitchell Guttman

Yuin-Han Loh

Kelly Thomas

In Hyun Park

Manuel Garber

Matthew Curran

Tamer Onder

Suneet Agarwal

Philip D Manos

Sumon Datta

Eric S Lander

Thorsten M Schlaeger

George Q Daley

John L Rinn

Affiliations

Soon will be listed here.

Abstract

The conversion of lineage-committed cells to induced pluripotent stem cells (iPSCs) by reprogramming is accompanied by a global remodeling of the epigenome, resulting in altered patterns of gene expression. Here we characterize the transcriptional reorganization of large intergenic non-coding RNAs (lincRNAs) that occurs upon derivation of human iPSCs and identify numerous lincRNAs whose expression is linked to pluripotency. Among these, we defined ten lincRNAs whose expression was elevated in iPSCs compared with embryonic stem cells, suggesting that their activation may promote the emergence of iPSCs. Supporting this, our results indicate that these lincRNAs are direct targets of key pluripotency transcription factors. Using loss-of-function and gain-of-function approaches, we found that one such lincRNA (lincRNA-RoR) modulates reprogramming, thus providing a first demonstration for critical functions of lincRNAs in the derivation of pluripotent stem cells.

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