Cystic Echinococcosis of Camels: 12S RRNA Gene Variation Revealed Changing Pattern of Genetic Diversity Within Sensu Lato in the Middle East and North/Sub-Saharan Africa

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2020 Oct 23

PMID 33094107

Citations 2

Authors

Mansoureh Dehghani

Mohammad Ali Mohammadi

Sahel Hemmati

Saeid Nasibi

Sima Rostami

Majid Fasihi Harandi

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis (CE) is one of the most widespread zoonotic diseases, with considerable public health and economic importance. Camels play a significant role in transmission cycle of especially, in the Middle East and North Africa (MENA). The present study aimed to identify the genetic variation and haplotype distribution of camel isolates of sensu lato using all existing mitochondrial DNA data from camels in different parts of the world. Sequence data from 1,144 camel isolates of s.l. available in the NCBI GenBank including 57 camel hydatid cysts collected in central Iran were used to analyze the nature of genetic variation within the camel isolates of s.l. in MENA region. Fifty-seven camel isolates were also PCR-sequenced on mitochondrial 12S rRNA gene. Haplotype network analysis revealed seven different haplotypes clustered into four major groups. G6 was identified as the most commonly represented genotype in camels followed by G1. Mitochondrial 12S rRNA gene sequence analysis on 57 camel isolates identified three different genotypes, including /G6 (35/57, 61.4%), sensu stricto/G1-G3 (21/57, 36.8%) as well as one isolate identified as /G5 (1/57, 1.8%). The number of base substitutions per site over 420 positions of partial 12S rRNA gene sequences were shown as 0.000 and 0.004 for (G6) corresponding to the Middle East and sub-Saharan isolates, respectively. Camel isolates of in the MENA region present moderate genetic diversity (Hd = 0.5540-0.6050). The Middle East isolates demonstrated a more diverse population than the North/sub-Saharan isolates, where six out of seven 12S rRNA haplotypes were identified in the former region. (G6 genotype) was shown to be the most common species in the world camel population. In conclusion, camels showed to be an important intermediate host species in the MENA region with different patterns of genetic variation between the Middle East and Africa.

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