The Role of Cattle in the Epidemiology of Echinococcus Granulosus in an Endemic Area of Southern Italy

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2008 Mar 14

PMID 18338180

Citations 17

Authors

L Rinaldi

M P Maurelli

V Veneziano

F Capuano

A G Perugini

S Cringoli

Affiliations

Soon will be listed here.

Abstract

An epidemiological and molecular survey was conducted to investigate the role of cattle in the transmission chain of cystic echinococcosis (CE) in the Campania region of southern Italy. Out of a total of 434 cattle examined for CE, 45 (10.4%) were found infected. A total of 363 cysts were collected from the infected animals: 239 in the liver and 124 in the lungs. The cysts were either sterile (42.7%) or calcified/caseous (57.3%); no fertile cysts were found. Most of the cysts had sizes <3 cm (77.1%) and were unilocular (78.8%). The results of the linear regression model did not show any significant correlation between the age of infected cattle and the number of cysts. The sequencing of the mitochondrial cytochrome C oxidase subunit 1 (CO1) gene of 40 hydatid cysts produced sequences of 419 bp for each sample analyzed. Alignment of the obtained sequences with those present in GenBank showed 100% identity with the common sheep G1 (n=21 cysts), the Tasmanian sheep G2 (n=2 cysts), and the buffalo G3 (n=17 cysts) strains, which constitute the species Echinococcus granulosus sensu stricto. The findings reported in the present study show that CE is widespread in cattle bred in the Campania region of southern Italy. However, the absence of fertile cysts and of the cattle strain (G5, E. ortleppi) suggests that cattle would not have any role in the persistence of this important zoonosis but rather a role as indicators of CE infection in this endemic area.

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References

Eckert J, Thompson R . Intraspecific variation of Echinococcus granulosus and related species with emphasis on their infectivity to humans. Acta Trop. 1997; 64(1-2):19-34. DOI: 10.1016/s0001-706x(96)00635-3. View

Rinaldi L, Maurelli M, Capuano F, Perugini A, Veneziano V, Cringoli S . Molecular update on cystic echinococcosis in cattle and water buffaloes of southern Italy. Zoonoses Public Health. 2008; 55(2):119-23. DOI: 10.1111/j.1863-2378.2007.01101.x. View

Mattapallil M, Ali S . Analysis of conserved microsatellite sequences suggests closer relationship between water buffalo Bubalus bubalis and sheep Ovis aries. DNA Cell Biol. 1999; 18(6):513-9. DOI: 10.1089/104454999315231. View

Capuano F, Rinaldi L, Maurelli M, Perugini A, Veneziano V, Garippa G . Cystic echinococcosis in water buffaloes: epidemiological survey and molecular evidence of ovine (G1) and buffalo (G3) strains. Vet Parasitol. 2006; 137(3-4):262-8. DOI: 10.1016/j.vetpar.2006.01.016. View

Craig P, McManus D, Lightowlers M, Chabalgoity J, Garcia H, Gavidia C . Prevention and control of cystic echinococcosis. Lancet Infect Dis. 2007; 7(6):385-94. DOI: 10.1016/S1473-3099(07)70134-2. View

Dalimi A, Motamedi G, Hosseini M, Mohammadian B, Malaki H, Ghamari Z . Echinococcosis/hydatidosis in western Iran. Vet Parasitol. 2002; 105(2):161-71. DOI: 10.1016/s0304-4017(02)00005-5. View

Romig T, Dinkel A, Mackenstedt U . The present situation of echinococcosis in Europe. Parasitol Int. 2005; 55 Suppl:S187-91. DOI: 10.1016/j.parint.2005.11.028. View

Seimenis A, Morelli D, Mantovani A . Zoonoses in the Mediterranean region. Ann Ist Super Sanita. 2007; 42(4):437-45. View

Banks D, Copeman D, Skerratt L, Molina E . Echinococcus granulosus in northern Queensland. 1. Prevalence in cattle. Aust Vet J. 2006; 84(9):303-7. DOI: 10.1111/j.1751-0813.2006.00020.x. View

10.

Varcasia A, Canu S, Kogkos A, Pipia A, Scala A, Garippa G . Molecular characterization of Echinococcus granulosus in sheep and goats of Peloponnesus, Greece. Parasitol Res. 2007; 101(4):1135-9. DOI: 10.1007/s00436-007-0568-x. View

11.

Garippa G, Battelli G, Cringoli G, Giangaspero A, Giannetto S, Manfredi M . [Animal echinococcosis in Italy: epidemiological update]. Parassitologia. 2004; 46(1-2):33-8. View

12.

Azlaf R, Dakkak A . Epidemiological study of the cystic echinococcosis in Morocco. Vet Parasitol. 2006; 137(1-2):83-93. DOI: 10.1016/j.vetpar.2006.01.003. View

13.

McManus D, Thompson R . Molecular epidemiology of cystic echinococcosis. Parasitology. 2004; 127 Suppl:S37-51. DOI: 10.1017/s0031182003003524. View

14.

Seimenis A . Overview of the epidemiological situation on echinococcosis in the Mediterranean region. Acta Trop. 2003; 85(2):191-5. DOI: 10.1016/s0001-706x(02)00272-3. View

15.

Nakao M, McManus D, Schantz P, Craig P, Ito A . A molecular phylogeny of the genus Echinococcus inferred from complete mitochondrial genomes. Parasitology. 2006; 134(Pt 5):713-22. DOI: 10.1017/S0031182006001934. View

16.

Thompson R, McManus D . Towards a taxonomic revision of the genus Echinococcus. Trends Parasitol. 2002; 18(10):452-7. DOI: 10.1016/s1471-4922(02)02358-9. View

17.

Romig T . Epidemiology of echinococcosis. Langenbecks Arch Surg. 2003; 388(4):209-17. DOI: 10.1007/s00423-003-0413-3. View

18.

Giannetto S, Poglayen G, Brianti E, Sorgi C, Gaglio G, Canu S . An epidemiological updating on cystic echinococcosis in cattle and sheep in Sicily, Italy. Parassitologia. 2005; 46(4):423-4. View

19.

Khan A, el-Buni A, Ali M . Fertility of the cysts of Echinococcus granulosus in domestic herbivores from Benghazi, Libya, and the reactivity of antigens produced from them. Ann Trop Med Parasitol. 2001; 95(4):337-42. DOI: 10.1080/00034980120053258. View

20.

Scala A, Canu S, Tanda B, Basciu M, Polinas L, Coccone G . An epidemiological and biomolecular survey of cystic echinococcosis in cattle in Sardinia. Parassitologia. 2005; 46(4):443-4. View