Prospective Isolation of Clonogenic Mantle Cell Lymphoma-initiating Cells

Overview

Journal Stem Cell Res

Publisher Elsevier

Specialty Cell Biology

Date 2010 Sep 21

PMID 20851072

Citations 15

Authors

Zheng Chen

Paul Ayala

Michael Wang

Luis Fayad

Ruth L Katz

Jorge Romaguera

Nancy Caraway

Sattva S Neelapu

Larry W Kwak

Paul J Simmons

Nami McCarty

Affiliations

Soon will be listed here.

Abstract

Here, we have prospectively isolated and characterized, for the first time, clonogenic cells with self-renewal capacities from mantle cell lymphoma (MCL), a particularly deadly form of non-Hodgkin's lymphoma (NHL). Self-renewal and tumorigenic activities were enriched in MCL cell fractions that lacked expression of the prototypic B-cell surface marker, CD19. CD45+CD19- cells represented a relatively small fraction of the total MCL tumor cells; however, they recapitulated the heterogeneity of original patient tumors on transplantation into immunodeficient mice. As few as 100 of these cells displayed self-renewal capacities in secondary and tertiary recipient mice by in vivo limiting dilution assays. Similar to leukemic stem cells, CD45+CD19- MCL cells also displayed a quiescent status as determined by dye efflux assays. In summary, this study is the first to isolate subpopulations of MCL cells that have self-renewal and tumorigenic capacities. Identification and characterization of MCL-ICs are important first steps toward understanding how self-renewal and tumorigenicity are regulated in MCL and designing targeted therapies against MCL-ICs will ultimately lead to improved outcomes for MCL patients.

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