Inflammatory Dendritic Cells--not Basophils--are Necessary and Sufficient for Induction of Th2 Immunity to Inhaled House Dust Mite Allergen

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2010 Sep 8

PMID 20819925

Citations 258

Authors

Hamida Hammad

Maud Plantinga

Kim Deswarte

Philippe Pouliot

Monique A M Willart

Mirjam Kool

Femke Muskens

Bart N Lambrecht

Affiliations

Soon will be listed here.

Abstract

It is unclear how Th2 immunity is induced in response to allergens like house dust mite (HDM). Here, we show that HDM inhalation leads to the TLR4/MyD88-dependent recruitment of IL-4 competent basophils and eosinophils, and of inflammatory DCs to the draining mediastinal nodes. Depletion of basophils only partially reduced Th2 immunity, and depletion of eosinophils had no effect on the Th2 response. Basophils did not take up inhaled antigen, present it to T cells, or express antigen presentation machinery, whereas a population of FceRI(+) DCs readily did. Inflammatory DCs were necessary and sufficient for induction of Th2 immunity and features of asthma, whereas basophils were not required. We favor a model whereby DCs initiate and basophils amplify Th2 immunity to HDM allergen.

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