Hemagglutinin of Influenza Virus Partitions into the Nonraft Domain of Model Membranes

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2010 Jul 21

PMID 20643067

Citations 28

Authors

Jorg Nikolaus

Silvia Scolari

Elisa Bayraktarov

Nadine Jungnick

Stephanie Engel

Anna Pia Plazzo

Martin Stockl

Rudolf Volkmer

Michael Veit

Andreas Herrmann

Affiliations

Soon will be listed here.

Abstract

The HA of influenza virus is a paradigm for a transmembrane protein thought to be associated with membrane-rafts, liquid-ordered like nanodomains of the plasma membrane enriched in cholesterol, glycosphingolipids, and saturated phospholipids. Due to their submicron size in cells, rafts can not be visualized directly and raft-association of HA was hitherto analyzed by indirect methods. In this study, we have used GUVs and GPMVs, showing liquid disordered and liquid ordered domains, to directly visualize partition of HA by fluorescence microscopy. We show that HA is exclusively (GUVs) or predominantly (GPMVs) present in the liquid disordered domain, regardless of whether authentic HA or domains containing its raft targeting signals were reconstituted into model membranes. The preferential partition of HA into ld domains and the difference between lo partition in GUV and GPMV are discussed with respect to differences in packaging of lipids in membranes of model systems and living cells suggesting that physical properties of lipid domains in biological membranes are tightly regulated by protein-lipid interactions.

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