Hemagglutinin Activation of Pathogenic Avian Influenza Viruses of Serotype H7 Requires the Protease Recognition Motif R-X-K/R-R

Overview

Journal Virology

Specialty Microbiology

Date 1992 May 1

PMID 1566583

Citations 56

Authors

M Vey

M Orlich

S Adler

H D Klenk

R Rott

W Garten

Affiliations

Soon will be listed here.

Abstract

The hemagglutinin of influenza virus A/FPV/Rostock/34 (H7) was altered at its multibasic cleavage site by site-directed mutagenesis and assayed for proteolytic activation after expression in CV-1 cells. The results indicated that the cellular protease responsible for activation recognizes the tetrapeptide motif R-X-K/R-R that must be presented in the correct sequence position. Studies on plaque variants of influenza virus A/fowl/Victoria/75 (H7N7) showed that alteration of the consensus sequence resulted in a loss of pathogenicity for chickens.

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