Accumulation of Raft Lipids in T-cell Plasma Membrane Domains Engaged in TCR Signalling

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2009 Jan 30

PMID 19177148

Citations 134

Authors

Tobias Zech

Christer S Ejsing

Katharina Gaus

Ben de Wet

Andrej Shevchenko

Kai Simons

Thomas Harder

Affiliations

Soon will be listed here.

Abstract

Activating stimuli for T lymphocytes are transmitted through plasma membrane domains that form at T-cell antigen receptor (TCR) signalling foci. Here, we determined the molecular lipid composition of immunoisolated TCR activation domains. We observed that they accumulate cholesterol, sphingomyelin and saturated phosphatidylcholine species as compared with control plasma membrane fragments. This provides, for the first time, direct evidence that TCR activation domains comprise a distinct molecular lipid composition reminiscent of liquid-ordered raft phases in model membranes. Interestingly, TCR activation domains were also enriched in plasmenyl phosphatidylethanolamine and phosphatidylserine. Modulating the T-cell lipidome with polyunsaturated fatty acids impaired the plasma membrane condensation at TCR signalling foci and resulted in a perturbed molecular lipid composition. These results correlate the accumulation of specific molecular lipid species with the specific plasma membrane condensation at sites of TCR activation and with early TCR activation responses.

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