QIP, a Protein That Converts Duplex SiRNA into Single Strands, is Required for Meiotic Silencing by Unpaired DNA

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2010 Jun 17

PMID 20551436

Citations 24

Authors

Hua Xiao

William G Alexander

Thomas M Hammond

Erin C Boone

Tony D Perdue

Patricia J Pukkila

Patrick K T Shiu

Affiliations

Soon will be listed here.

Abstract

RNA interference (RNAi) depends on the production of small RNA to regulate gene expression in eukaryotes. Two RNAi systems exist to control repetitive selfish elements in Neurospora crassa. Quelling targets transgenes during vegetative growth, whereas meiotic silencing by unpaired DNA (MSUD) silences unpaired genes during meiosis. The two mechanisms require common RNAi proteins, such as RNA-directed RNA polymerases, Dicers, and Argonaute slicers. We have previously demonstrated that, while Quelling depends on the redundant dicer activity of DCL-1 and DCL-2, only DCL-1 is required for MSUD. Here, we show that QDE-2-interacting protein (QIP), an exonuclease that is important for the production of single-stranded siRNA during Quelling, is also required for MSUD. QIP is crucial for sexual development and is shown to colocalize with other MSUD proteins in the perinuclear region.

Citing Articles

A DEAD-box RNA helicase mediates meiotic silencing by unpaired DNA.

Sy V, Boone E, Xiao H, Vierling M, Schmitz S, Ung Q G3 (Bethesda). 2023; 13(8).

PMID: 37052947 PMC: 10411587. DOI: 10.1093/g3journal/jkad083.

Disruption of the RNA interference machinery alters the conidial transcriptome.

Kelani A, Bruch A, Rivieccio F, Visser C, Kruger T, Weaver D RNA. 2023; 29(7):1033-1050.

PMID: 37019633 PMC: 10275271. DOI: 10.1261/rna.079350.122.

Involvement of RNA granule proteins in meiotic silencing by unpaired DNA.

Xiao H, Vierling M, Kennedy R, Boone E, Decker L, Sy V G3 (Bethesda). 2021; 11(10).

PMID: 34568932 PMC: 8482848. DOI: 10.1093/g3journal/jkab179.

Role of the Non-Canonical RNAi Pathway in the Antifungal Resistance and Virulence of Mucorales.

Canovas-Marquez J, Navarro-Mendoza M, Perez-Arques C, Lax C, Tahiri G, Perez-Ruiz J Genes (Basel). 2021; 12(4).

PMID: 33920552 PMC: 8072676. DOI: 10.3390/genes12040586.

An NCBP3-Domain Protein Mediates Meiotic Silencing by Unpaired DNA.

Boone E, Xiao H, Vierling M, Decker L, Sy V, Kennedy R G3 (Bethesda). 2020; 10(6):1919-1927.

PMID: 32291289 PMC: 7263685. DOI: 10.1534/g3.120.401236.

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