RISC-interacting Clearing 3'- 5' Exoribonucleases (RICEs) Degrade Uridylated Cleavage Fragments to Maintain Functional RISC in

Overview

Journal Elife

Specialty Biology

Date 2017 May 3

PMID 28463111

Citations 29

Authors

Zhonghui Zhang

Fuqu Hu

Min Woo Sung

Chang Shu

Claudia Castillo-Gonzalez

Hisashi Koiwa

Guiliang Tang

Martin Dickman

Pingwei Li

Xiuren Zhang

Affiliations

Soon will be listed here.

Abstract

RNA-induced silencing complex (RISC) is composed of miRNAs and AGO proteins. AGOs use miRNAs as guides to slice target mRNAs to produce truncated 5' and 3' RNA fragments. The 5' cleaved RNA fragments are marked with uridylation for degradation. Here, we identified novel cofactors of Arabidopsis AGOs, named RICE1 and RICE2. RICE proteins specifically degraded single-strand (ss) RNAs in vitro; but neither miRNAs nor miRNA*s in vivo. RICE1 exhibited a DnaQ-like exonuclease fold and formed a homohexamer with the active sites located at the interfaces between RICE1 subunits. Notably, ectopic expression of catalytically-inactive RICE1 not only significantly reduced miRNA levels; but also increased 5' cleavage RISC fragments with extended uridine tails. We conclude that RICEs act to degrade uridylated 5' products of AGO cleavage to maintain functional RISC. Our study also suggests a possible link between decay of cleaved target mRNAs and miRNA stability in RISC.

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