An NCBP3-Domain Protein Mediates Meiotic Silencing by Unpaired DNA

Overview

Journal G3 (Bethesda)

Publisher Oxford University Press

Specialties Genetics
Molecular Biology

Date 2020 Apr 16

PMID 32291289

Citations 2

Authors

Erin C Boone

Hua Xiao

Michael M Vierling

Logan M Decker

Victor T Sy

Rana F Kennedy

Marilyn A Bonham

Shannon F Schmitz

Annie M John

Thomas M Hammond

Patrick K T Shiu

Affiliations

Soon will be listed here.

Abstract

In the filamentous fungus , genes unpaired during meiosis are silenced by a process known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) proteins, such as Dicer and Argonaute, to target homologous mRNAs for silencing. Previously, we demonstrated that nuclear cap-binding proteins NCBP1 and NCBP2 are involved in MSUD. We report here that SAD-8, a protein similar to human NCBP3, also mediates silencing. Although SAD-8 is not essential for either vegetative or sexual development, it is required for MSUD. SAD-8 localizes predominantly in the nucleus and interacts with both NCBP1 and NCBP2. Similar to NCBP1 and NCBP2, SAD-8 interacts with a component (Argonaute) of the perinuclear meiotic silencing complex (MSC), further implicating the involvement of cap-binding proteins in silencing.

Citing Articles

A DEAD-box RNA helicase mediates meiotic silencing by unpaired DNA.

Sy V, Boone E, Xiao H, Vierling M, Schmitz S, Ung Q G3 (Bethesda). 2023; 13(8).

PMID: 37052947 PMC: 10411587. DOI: 10.1093/g3journal/jkad083.

Involvement of RNA granule proteins in meiotic silencing by unpaired DNA.

Xiao H, Vierling M, Kennedy R, Boone E, Decker L, Sy V G3 (Bethesda). 2021; 11(10).

PMID: 34568932 PMC: 8482848. DOI: 10.1093/g3journal/jkab179.

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