Abundant Ribonucleotide Incorporation into DNA by Yeast Replicative Polymerases

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2010 Mar 3

PMID 20194773

Citations 261

Authors

Stephanie A Nick McElhinny

Brian E Watts

Dinesh Kumar

Danielle L Watt

Else-Britt Lundstrom

Peter M J Burgers

Erik Johansson

Andrei Chabes

Thomas A Kunkel

Affiliations

Soon will be listed here.

Abstract

Measurements of nucleoside triphosphate levels in Saccharomyces cerevisiae reveal that the four rNTPs are in 36- to 190-fold molar excess over their corresponding dNTPs. During DNA synthesis in vitro using the physiological nucleoside triphosphate concentrations, yeast DNA polymerase epsilon, which is implicated in leading strand replication, incorporates one rNMP for every 1,250 dNMPs. Pol delta and Pol alpha, which conduct lagging strand replication, incorporate one rNMP for every 5,000 or 625 dNMPs, respectively. Discrimination against rNMP incorporation varies widely, in some cases by more than 100-fold, depending on the identity of the base and the template sequence context in which it is located. Given estimates of the amount of replication catalyzed by Pols alpha, delta, and epsilon, the results are consistent with the possibility that more than 10,000 rNMPs may be incorporated into the nuclear genome during each round of replication in yeast. Thus, rNMPs may be the most common noncanonical nucleotides introduced into the eukaryotic genome. Potential beneficial and negative consequences of abundant ribonucleotide incorporation into DNA are discussed, including the possibility that unrepaired rNMPs in DNA could be problematic because yeast DNA polymerase epsilon has difficulty bypassing a single rNMP present within a DNA template.

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