CD8+ Lymphocytes Control Viral Replication in SIVmac239-infected Rhesus Macaques Without Decreasing the Lifespan of Productively Infected Cells

Overview

Journal PLoS Pathog

Specialty Microbiology

Date 2010 Feb 4

PMID 20126441

Citations 123

Authors

Nichole R Klatt

Emi Shudo

Alex M Ortiz

Jessica C Engram

Mirko Paiardini

Benton Lawson

Michael D Miller

James Else

Ivona Pandrea

Jacob D Estes

Cristian Apetrei

Joern E Schmitz

Ruy M Ribeiro

Alan S Perelson

Guido Silvestri

Affiliations

Soon will be listed here.

Abstract

While CD8+ T cells are clearly important in controlling virus replication during HIV and SIV infections, the mechanisms underlying this antiviral effect remain poorly understood. In this study, we assessed the in vivo effect of CD8+ lymphocyte depletion on the lifespan of productively infected cells during chronic SIVmac239 infection of rhesus macaques. We treated two groups of animals that were either CD8+ lymphocyte-depleted or controls with antiretroviral therapy, and used mathematical modeling to assess the lifespan of infected cells either in the presence or absence of CD8+ lymphocytes. We found that, in both early (day 57 post-SIV) and late (day 177 post-SIV) chronic SIV infection, depletion of CD8+ lymphocytes did not result in a measurable increase in the lifespan of either short- or long-lived productively infected cells in vivo. This result indicates that the presence of CD8+ lymphocytes does not result in a noticeably shorter lifespan of productively SIV-infected cells, and thus that direct cell killing is unlikely to be the main mechanism underlying the antiviral effect of CD8+ T cells in SIV-infected macaques with high virus replication.

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