Healthy Baby Born After Reduction of Sperm DNA Fragmentation Using Cell Sorting Before ICSI

Overview

Journal Reprod Biomed Online

Publisher Elsevier

Specialty Reproductive Medicine

Date 2010 Jan 26

PMID 20096634

Citations 14

Authors

Vanesa Y Rawe

Heydy Uriondo Boudri

Cristian Alvarez Sedo

Martina Carro

Sergio Papier

Florencia Nodar

Affiliations

Soon will be listed here.

Abstract

Magnetic activated cell sorting (MACS) with annexin V microbeads recognizes externalized phosphatidylserine (PS) residues on the surface of apoptotic spermatozoa. The successful use of this novel technique applied to a highly apoptotic semen sample before performing intracytoplasmic sperm injection (ICSI) is reported here. The use of annexin V microbeads for selecting non-apoptotic spermatozoa seems to reduce the percentage of altered cells, improving the chance of pregnancy after ICSI.

Citing Articles

The Use of Non-Apoptotic Sperm Selected by Magnetic Activated Cell Sorting (MACS) to Enhance Reproductive Outcomes: What the Evidence Says.

Garrido N, Gil Julia M Biology (Basel). 2024; 13(1).

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MACS-annexin V cell sorting of semen samples with high TUNEL values decreases the concentration of cells with abnormal chromosomal content: a pilot study.

El Fekih S, Gueganic N, Tous C, Ben Ali H, Ajina M, Douet-Guilbert N Asian J Androl. 2022; 24(5):445-450.

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Magnetic-activated cell sorting of nonapoptotic spermatozoa with a high DNA fragmentation index improves the live birth rate and decreases transfer cycles of IVF/ICSI.

Mei J, Chen L, Zhu X, Yu W, Gao Q, Sun H Asian J Androl. 2021; 24(4):367-372.

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Sperm DNA Fragmentation: A New Guideline for Clinicians.

Agarwal A, Majzoub A, Baskaran S, Panner Selvam M, Cho C, Henkel R World J Mens Health. 2020; 38(4):412-471.

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Magnetic-activated cell sorting of non-apoptotic spermatozoa improves the quality of embryos according to female age: a prospective sibling oocyte study.

Stimpfel M, Verdenik I, Zorn B, Virant-Klun I J Assist Reprod Genet. 2018; 35(9):1665-1674.

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