Label-free Cellular Manipulation and Sorting Via Biocompatible Ferrofluids

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Dec 10

PMID 19995975

Citations 41

Authors

Ayse R Kose

Birgit Fischer

Leidong Mao

Hur Koser

Affiliations

Soon will be listed here.

Abstract

We present a simple microfluidic platform that uses biocompatible ferrofluids for the controlled manipulation and rapid separation of both microparticles and live cells. This low-cost platform exploits differences in particle size, shape, and elasticity to achieve rapid and efficient separation. Using microspheres, we demonstrate size-based separation with 99% separation efficiency and sub-10-microm resolution in <45 s. We also show continuous manipulation and shape-based separation of live red blood cells from sickle cells and bacteria. These initial demonstrations reveal the potential of ferromicrofluidics in significantly reducing incubation times and increasing diagnostic sensitivity in cellular assays through rapid separation and delivery of target cells to sensor arrays.

Citing Articles

Integrating conductive electrodes into hydrogel-based microfluidic chips for real-time monitoring of cell response.

Pourmostafa A, Bhusal A, Haridas Menon N, Li Z, Basuray S, Miri A Front Bioeng Biotechnol. 2024; 12:1421592.

PMID: 39257447 PMC: 11384590. DOI: 10.3389/fbioe.2024.1421592.

Design and Development of a Traveling Wave Ferro-Microfluidic Device and System Rig for Potential Magnetophoretic Cell Separation and Sorting in a Water-Based Ferrofluid.

Hewlin Jr R, Edwards M, Schultz C Micromachines (Basel). 2023; 14(4).

PMID: 37421122 PMC: 10145302. DOI: 10.3390/mi14040889.

Shaping Liquid Droplets on an Active Air-Ferrofluid Interface.

Harischandra P, Valisalmi T, Cenev Z, Linder M, Zhou Q Langmuir. 2023; 39(22):7623-7631.

PMID: 37224278 PMC: 10249407. DOI: 10.1021/acs.langmuir.3c00298.

Fabrication and Manipulation of Non-Spherical Particles in Microfluidic Channels: A Review.

Jiang D, Liu S, Tang W Micromachines (Basel). 2022; 13(10).

PMID: 36296012 PMC: 9611947. DOI: 10.3390/mi13101659.

From Single-Core Nanoparticles in Ferrofluids to Multi-Core Magnetic Nanocomposites: Assembly Strategies, Structure, and Magnetic Behavior.

Krasia-Christoforou T, Socoliuc V, Knudsen K, Tombacz E, Turcu R, Vekas L Nanomaterials (Basel). 2020; 10(11).

PMID: 33142887 PMC: 7692798. DOI: 10.3390/nano10112178.

References

Lekka M, Laidler P, Gil D, Lekki J, Stachura Z, Hrynkiewicz A . Elasticity of normal and cancerous human bladder cells studied by scanning force microscopy. Eur Biophys J. 1999; 28(4):312-6. DOI: 10.1007/s002490050213. View

Muller T, Pfennig A, Klein P, Gradl G, Jager M, Schnelle T . The potential of dielectrophoresis for single-cell experiments. IEEE Eng Med Biol Mag. 2004; 22(6):51-61. DOI: 10.1109/memb.2003.1266047. View

Cabrera C, Yager P . Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques. Electrophoresis. 2001; 22(2):355-62. DOI: 10.1002/1522-2683(200101)22:2<355::AID-ELPS355>3.0.CO;2-C. View

Liu Y, Cheng D, Sonek G, Berns M, Chapman C, Tromberg B . Evidence for localized cell heating induced by infrared optical tweezers. Biophys J. 1995; 68(5):2137-44. PMC: 1282119. DOI: 10.1016/S0006-3495(95)80396-6. View

Yellen B, Hovorka O, Friedman G . Arranging matter by magnetic nanoparticle assemblers. Proc Natl Acad Sci U S A. 2005; 102(25):8860-4. PMC: 1150276. DOI: 10.1073/pnas.0500409102. View

Applegate Jr R, Squier J, Vestad T, Oakey J, Marr D . Optical trapping, manipulation, and sorting of cells and colloids in microfluidic systems with diode laser bars. Opt Express. 2009; 12(19):4390-8. DOI: 10.1364/opex.12.004390. View

Mao L, Koser H . Towards ferrofluidics for μ-TAS and lab on-a-chip applications. Nanotechnology. 2011; 17(4):S34-47. DOI: 10.1088/0957-4484/17/4/007. View

Dittrich P, Manz A . Lab-on-a-chip: microfluidics in drug discovery. Nat Rev Drug Discov. 2006; 5(3):210-8. DOI: 10.1038/nrd1985. View

Pethig R, Markx G . Applications of dielectrophoresis in biotechnology. Trends Biotechnol. 1997; 15(10):426-32. DOI: 10.1016/S0167-7799(97)01096-2. View

10.

Ashkin A, Dziedzic J, Yamane T . Optical trapping and manipulation of single cells using infrared laser beams. Nature. 1987; 330(6150):769-71. DOI: 10.1038/330769a0. View

11.

Ashkin A, Dziedzic J . Optical trapping and manipulation of viruses and bacteria. Science. 1987; 235(4795):1517-20. DOI: 10.1126/science.3547653. View

12.

Menachery A, Pethig R . Controlling cell destruction using dielectrophoretic forces. IEE Proc Nanobiotechnol. 2006; 152(4):145-9. DOI: 10.1049/ip-nbt:20050010. View

13.

Cheong K, Yi D, Lee J, Park J, Kim M, Edel J . Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber. Lab Chip. 2008; 8(5):810-3. DOI: 10.1039/b717382b. View

14.

Yan J, Skoko D, Marko J . Near-field-magnetic-tweezer manipulation of single DNA molecules. Phys Rev E Stat Nonlin Soft Matter Phys. 2004; 70(1 Pt 1):011905. DOI: 10.1103/PhysRevE.70.011905. View

15.

Blattner F, Plunkett 3rd G, Bloch C, Perna N, Burland V, Riley M . The complete genome sequence of Escherichia coli K-12. Science. 1997; 277(5331):1453-62. DOI: 10.1126/science.277.5331.1453. View

16.

Kremser L, Blaas D, Kenndler E . Capillary electrophoresis of biological particles: viruses, bacteria, and eukaryotic cells. Electrophoresis. 2004; 25(14):2282-91. DOI: 10.1002/elps.200305868. View

17.

Chiou P, Ohta A, Wu M . Massively parallel manipulation of single cells and microparticles using optical images. Nature. 2005; 436(7049):370-2. DOI: 10.1038/nature03831. View

18.

Davis J, Inglis D, Morton K, Lawrence D, Huang L, Chou S . Deterministic hydrodynamics: taking blood apart. Proc Natl Acad Sci U S A. 2006; 103(40):14779-84. PMC: 1595428. DOI: 10.1073/pnas.0605967103. View

19.

Hughes M . Strategies for dielectrophoretic separation in laboratory-on-a-chip systems. Electrophoresis. 2002; 23(16):2569-82. DOI: 10.1002/1522-2683(200208)23:16<2569::AID-ELPS2569>3.0.CO;2-M. View

20.

Kamei T, Toriello N, Lagally E, Blazej R, Scherer J, Street R . Microfluidic genetic analysis with an integrated a-Si:H detector. Biomed Microdevices. 2005; 7(2):147-52. DOI: 10.1007/s10544-005-1595-y. View