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Deterministic Hydrodynamics: Taking Blood Apart

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 2006 Sep 27
PMID 17001005
Citations 138
Authors
John A Davis
David W Inglis
Keith J Morton
David A Lawrence
Lotien R Huang
Stephen Y Chou
James C Sturm
Robert H Austin
Affiliations
Soon will be listed here.
Abstract

We show the fractionation of whole blood components and isolation of blood plasma with no dilution by using a continuous-flow deterministic array that separates blood components by their hydrodynamic size, independent of their mass. We use the technology we developed of deterministic arrays which separate white blood cells, red blood cells, and platelets from blood plasma at flow velocities of 1,000 microm/sec and volume rates up to 1 microl/min. We verified by flow cytometry that an array using focused injection removed 100% of the lymphocytes and monocytes from the main red blood cell and platelet stream. Using a second design, we demonstrated the separation of blood plasma from the blood cells (white, red, and platelets) with virtually no dilution of the plasma and no cellular contamination of the plasma.

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