Yeast Cell Fractionation by Morphology in Dilute Ferrofluids

Overview

Journal Biomicrofluidics

Publisher American Institute of Physics

Specialty Biomedical Engineering

Date 2017 Nov 21

PMID 29152030

Citations 8

Authors

Qi Chen

Di Li

Jessica Zielinski

Lukasz Kozubowski

Jianhan Lin

Maohua Wang

Xiangchun Xuan

Affiliations

Soon will be listed here.

Abstract

Morphology is an important particle (both biological and synthetic) property and potentially a useful marker for label-free particle separation. We present in this work a continuous-flow morphology-based fractionation of a heterogeneous mixture of drug-treated yeast cells in dilute ferrofluids. Such a diamagnetic cell separation technique utilizes the negative magnetophoretic motion to direct pre-focused yeast cells to morphology-dependent streamlines in a laminar flow. The separation performance is evaluated by comparing the exiting positions of the four classified groups of yeast cells: Singles, Doubles, Triples, and Others. We also develop a three-dimensional numerical model to simulate the separation process by the use of the experimentally determined correction factor for each group of non-spherical cells. The determining factors in this separation are studied both experimentally and numerically, the results of which show a reasonable agreement.

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