Contribution of the Multidrug Efflux Transporter CmeABC to Antibiotic Resistance in Different Campylobacter Species

Overview

Journal Foodborne Pathog Dis

Specialties Microbiology
Nutritional Sciences
Parasitology

Date 2009 Sep 30

PMID 19785541

Citations 19

Authors

Baoqing Guo

Jun Lin

Donald L Reynolds

Qijing Zhang

Affiliations

Soon will be listed here.

Abstract

CmeABC, a multidrug efflux system in Campylobacter jejuni, plays an important role in the resistance to different antimicrobials and toxic compounds. Although this efflux system has been well characterized in C. jejuni and to a less extent in C. coli, it is unknown if CmeABC homologs are functional in other Campylobacter spp. In this study, the cmeABC homologs were identified and functionally characterized in five Campylobacter species including C. jejuni, C. coli, C. lari, C. upsaliensis, and C. fetus. Our results indicated that cmeABC is present in all five Campylobacter spp. and the genomic organization of this efflux operon is similar among the Campylobacter spp. Insertional mutagenesis of cmeB increased the susceptibilities of all the five Campylobacter spp. to structurally diverse antimicrobials. Together, these results indicated that the CmeABC efflux system is conserved at both the genomic and functional levels in all five Campylobacter spp. examined in this study, further highlighting the significant role of CmeABC in Campylobacter pathobiology.

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References

Karmali M, de Grandis S, Fleming P . Antimicrobial susceptibility of Campylobacter jejuni with special reference to resistance patterns of Canadian isolates. Antimicrob Agents Chemother. 1981; 19(4):593-7. PMC: 181484. DOI: 10.1128/AAC.19.4.593. View

Luangtongkum T, Jeon B, Han J, Plummer P, Logue C, Zhang Q . Antibiotic resistance in Campylobacter: emergence, transmission and persistence. Future Microbiol. 2009; 4(2):189-200. PMC: 2691575. DOI: 10.2217/17460913.4.2.189. View

Cagliero C, Cloix L, Cloeckaert A, Payot S . High genetic variation in the multidrug transporter cmeB gene in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother. 2006; 58(1):168-72. DOI: 10.1093/jac/dkl212. View

Tee W, Mijch A . Campylobacter jejuni bacteremia in human immunodeficiency virus (HIV)-infected and non-HIV-infected patients: comparison of clinical features and review. Clin Infect Dis. 1998; 26(1):91-6. DOI: 10.1086/516263. View

Bourke B, Chan V, Sherman P . Campylobacter upsaliensis: waiting in the wings. Clin Microbiol Rev. 1998; 11(3):440-9. PMC: 88890. DOI: 10.1128/CMR.11.3.440. View

Corcoran D, Quinn T, Cotter L, OHalloran F, Fanning S . Characterization of a cmeABC operon in a quinolone-resistant Campylobacter coli isolate of Irish origin. Microb Drug Resist. 2005; 11(4):303-8. DOI: 10.1089/mdr.2005.11.303. View

Mamelli L, Prouzet-Mauleon V, Pages J, Megraud F, Bolla J . Molecular basis of macrolide resistance in Campylobacter: role of efflux pumps and target mutations. J Antimicrob Chemother. 2005; 56(3):491-7. DOI: 10.1093/jac/dki253. View

Guillaume G, Ledent V, Moens W, Collard J . Phylogeny of efflux-mediated tetracycline resistance genes and related proteins revisited. Microb Drug Resist. 2004; 10(1):11-26. DOI: 10.1089/107662904323047754. View

Ng L, Taylor D, Stiles M . Characterization of freshly isolated Campylobacter coli strains and suitability of selective media for their growth. J Clin Microbiol. 1988; 26(3):518-23. PMC: 266324. DOI: 10.1128/jcm.26.3.518-523.1988. View

10.

Goossens H, De Boeck M, Coignau H, Vlaes L, Van den Borre C, Butzler J . Modified selective medium for isolation of Campylobacter spp. from feces: comparison with Preston medium, a blood-free medium, and a filtration system. J Clin Microbiol. 1986; 24(5):840-3. PMC: 269038. DOI: 10.1128/jcm.24.5.840-843.1986. View

11.

Fakhr M, Logue C . Sequence variation in the outer membrane protein-encoding gene cmeC, conferring multidrug resistance among Campylobacter jejuni and Campylobacter coli strains isolated from different hosts. J Clin Microbiol. 2007; 45(10):3381-3. PMC: 2045336. DOI: 10.1128/JCM.01208-07. View

12.

Burnens A, Nicolet J . Detection of Campylobacter upsaliensis in diarrheic dogs and cats, using a selective medium with cefoperazone. Am J Vet Res. 1992; 53(1):48-51. View

13.

Lachance N, Gaudreau C, Lamothe F, Lariviere L . Role of the beta-lactamase of Campylobacter jejuni in resistance to beta-lactam agents. Antimicrob Agents Chemother. 1991; 35(5):813-8. PMC: 245112. DOI: 10.1128/AAC.35.5.813. View

14.

Piddock L . Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria. Clin Microbiol Rev. 2006; 19(2):382-402. PMC: 1471989. DOI: 10.1128/CMR.19.2.382-402.2006. View

15.

Cagliero C, Mouline C, Payot S, Cloeckaert A . Involvement of the CmeABC efflux pump in the macrolide resistance of Campylobacter coli. J Antimicrob Chemother. 2005; 56(5):948-50. DOI: 10.1093/jac/dki292. View

16.

Guerry P, Yao R, Alm R, Burr D, Trust T . Systems of experimental genetics for Campylobacter species. Methods Enzymol. 1994; 235:474-81. DOI: 10.1016/0076-6879(94)35163-5. View

17.

Lastovica A, Le Roux E . Efficient isolation of Campylobacter upsaliensis from stools. J Clin Microbiol. 2001; 39(11):4222-3. PMC: 88526. DOI: 10.1128/JCM.39.11.4222-4223.2001. View

18.

Allos B . Campylobacter jejuni Infections: update on emerging issues and trends. Clin Infect Dis. 2001; 32(8):1201-6. DOI: 10.1086/319760. View

19.

Lin J, Cagliero C, Guo B, Barton Y, Maurel M, Payot S . Bile salts modulate expression of the CmeABC multidrug efflux pump in Campylobacter jejuni. J Bacteriol. 2005; 187(21):7417-24. PMC: 1272998. DOI: 10.1128/JB.187.21.7417-7424.2005. View

20.

Lin J, Sahin O, Michel L, Zhang Q . Critical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejuni. Infect Immun. 2003; 71(8):4250-9. PMC: 165992. DOI: 10.1128/IAI.71.8.4250-4259.2003. View