Sequence Variation in the Outer Membrane Protein-encoding Gene CmeC, Conferring Multidrug Resistance Among Campylobacter Jejuni and Campylobacter Coli Strains Isolated from Different Hosts

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2007 Jul 27

PMID 17652470

Citations 8

Authors

Mohamed K Fakhr

Catherine M Logue

Affiliations

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Abstract

A novel PCR primer pair was used to detect the presence of cmeC in 131 Campylobacter jejuni and C. coli strains isolated from various hosts (cattle, turkeys, humans, and pigs). DNA sequence analysis revealed a high degree of genetic variation between the two species, while extremely limited genetic variation among isolates of the same species was detected.

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References

Van Bambeke F, Balzi E, Tulkens P . Antibiotic efflux pumps. Biochem Pharmacol. 2000; 60(4):457-70. DOI: 10.1016/s0006-2952(00)00291-4. View

Allos B . Campylobacter jejuni Infections: update on emerging issues and trends. Clin Infect Dis. 2001; 32(8):1201-6. DOI: 10.1086/319760. View

Dorrell N, Mangan J, Laing K, Hinds J, Linton D, Barrell B . Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity. Genome Res. 2001; 11(10):1706-15. PMC: 311159. DOI: 10.1101/gr.185801. View

Pumbwe L, Piddock L . Identification and molecular characterisation of CmeB, a Campylobacter jejuni multidrug efflux pump. FEMS Microbiol Lett. 2002; 206(2):185-9. DOI: 10.1111/j.1574-6968.2002.tb11007.x. View

Cloak O, Fratamico P . A multiplex polymerase chain reaction for the differentiation of Campylobacter jejuni and Campylobacter coli from a swine processing facility and characterization of isolates by pulsed-field gel electrophoresis and antibiotic resistance profiles. J Food Prot. 2002; 65(2):266-73. DOI: 10.4315/0362-028x-65.2.266. View

Lin J, Michel L, Zhang Q . CmeABC functions as a multidrug efflux system in Campylobacter jejuni. Antimicrob Agents Chemother. 2002; 46(7):2124-31. PMC: 127319. DOI: 10.1128/AAC.46.7.2124-2131.2002. View

Olah P, Doetkott C, Fakhr M, Logue C . Prevalence of the Campylobacter multi-drug efflux pump (CmeABC) in Campylobacter spp. Isolated from freshly processed Turkeys. Food Microbiol. 2006; 23(5):453-60. DOI: 10.1016/j.fm.2005.06.004. View

Lin J, Sahin O, Michel L, Zhang Q . Critical role of multidrug efflux pump CmeABC in bile resistance and in vivo colonization of Campylobacter jejuni. Infect Immun. 2003; 71(8):4250-9. PMC: 165992. DOI: 10.1128/IAI.71.8.4250-4259.2003. View

Charvalos E, Tselentis Y, Hamzehpour M, Kohler T, Pechere J . Evidence for an efflux pump in multidrug-resistant Campylobacter jejuni. Antimicrob Agents Chemother. 1995; 39(9):2019-22. PMC: 162874. DOI: 10.1128/AAC.39.9.2019. View

10.

Mead P, Slutsker L, Dietz V, McCaig L, Bresee J, Shapiro C . Food-related illness and death in the United States. Emerg Infect Dis. 1999; 5(5):607-25. PMC: 2627714. DOI: 10.3201/eid0505.990502. View

11.

Lin J, Akiba M, Sahin O, Zhang Q . CmeR functions as a transcriptional repressor for the multidrug efflux pump CmeABC in Campylobacter jejuni. Antimicrob Agents Chemother. 2005; 49(3):1067-75. PMC: 549222. DOI: 10.1128/AAC.49.3.1067-1075.2005. View

12.

Corcoran D, Quinn T, Cotter L, OHalloran F, Fanning S . Characterization of a cmeABC operon in a quinolone-resistant Campylobacter coli isolate of Irish origin. Microb Drug Resist. 2005; 11(4):303-8. DOI: 10.1089/mdr.2005.11.303. View

13.

Cagliero C, Cloix L, Cloeckaert A, Payot S . High genetic variation in the multidrug transporter cmeB gene in Campylobacter jejuni and Campylobacter coli. J Antimicrob Chemother. 2006; 58(1):168-72. DOI: 10.1093/jac/dkl212. View

14.

De Medici D, Croci L, Delibato E, Di Pasquale S, Filetici E, Toti L . Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry. Appl Environ Microbiol. 2003; 69(6):3456-61. PMC: 161507. DOI: 10.1128/AEM.69.6.3456-3461.2003. View